摘要
为了检测三磷酸腺苷(ATP)的浓度,通过Au-S键将巯基修饰的三磷酸腺苷的适体ssDNA自组装到薄膜金电极上,制备了一种可灵敏准确地检测ATP浓度的电流型适体传感器。首先研究了薄膜金电极的制备方法,接着研究了三磷酸腺苷的适体ssDNA在薄膜金电极上的组装方法,然后利用循环伏安法研究了自组装适体传感器在K3Fe(CN)6溶液中的响应电流与被测物ATP浓度之间的关系,最后对自组装适体传感器的线性度和重复性进行了分析。实验结果表明:自组装的电流型适体传感器在检测ATP浓度时,传感器的检出限为109 mol/L,线性范围10-9～10-5 mol/L,线性度为0.996 7。自组装的ATP适体传感器检出限低,线性检测范围宽,重复性好。
The aptamer ssDNA of the adenosine triphosphate （ATP） labeled with sulfydryl was self-assembled on the thin-film gold electrode by Au-S, and the amperometric aptasensor was fab- ricated sensitively and accurately to measure the concentration of the ATP. Firstly, the fabrica- tion method of the thin-film gold electrode was researched, and the self-assembled method of the ATP aptamers ssDNA on the thin-film gold electrode was presented. Then the relationship be- tween the response current of the self-assembled aptasensor and the concentration of the ATP in K3Fe（CN）6 solution were obtained with the cyclic voltammetry. Finally, the linearity and repea- tability of the self-assembled aptasensor were analyzed. The experiments indicate that the detec- tion limit is 10^-9 tool/L, the linearity range is 10^-9- 10^-5mol/L, and the linearity is 0. 996 7 when the ATP concentration is measured with the self-assembled amperometric aptasensor. The self-assembled aptasensor for the ATP has the characteristics of low detection limit, wide detec- tion range and good repeatability.
出处
《微纳电子技术》
CAS
北大核心
2013年第6期371-375,共5页
Micronanoelectronic Technology
基金
国家自然科学基金资助项目(61002007)
河南省科技创新人才计划资助项目(124100510001)
关键词
三磷酸腺苷
电流型适体传感器
薄膜金电极
循环伏安法
自组装
adenosine triphosphate
amperometric aptasensor
thin-film gold electrode
cyclic voltammetry
self-assembly
