摘要
[目的]探讨miR-143及其前体转录本在宫颈鳞癌中的表达及与临床病理特征的关系。[方法]采用Real-time PCR方法检测48例宫颈鳞癌及20例正常宫颈组织中miR-143的表达,分析miR-143与其前体转录本表达间的相关性,以及miR-143表达与宫颈鳞癌临床病理特征的关系。[结果]用Real-time PCR能有效检测miR-143及其前体转录本;miR-143及其前体转录本在宫颈鳞癌组织中的表达均显著低于正常宫颈组织(P<0.05),Spearman相关分析显示,miR-143前体转录本表达和miR-143表达呈明显正相关(r=0.723,P=0.000)。不同组织分化程度的宫颈鳞癌miR-143表达存在显著差异(P=0.005),小细胞型宫颈癌的miR-143表达明显低于其他组织分化类型的标本。miR-143的表达在不同年龄、肿块大小、大体类型及FIGO分期间未见显著差异(P>0.05)。[结论]前体转录本减少是miR-143表达下调的重要原因。miR-143在宫颈鳞癌组织中的异常表达,可能在宫颈鳞癌的发生和发展过程中发挥重要作用,有望成为宫颈鳞癌新的预后指标及治疗靶点。
[Purpose] To investigate the expressions of miR-143 and its primary transcript（pri-miR-143） in squamous cell carcinoma of cervix and the relationship of miR-143 with clinicopathologic features of squamous cell carcinoma of cervix.[Methods] The expressions of miR-143 in 48 cases with squamous cell carcinoma of cervix and 20 normal cervical tissue specimens were examined by real-time PCR.The correlation between the expressions of miR-143 and pri-miR-143 was analyzed.The relationship of the expression of miR-143 and clinicopathologic features of squamous cell carcinoma were explored.[Results] The real-time PCR could sensitively and specifically detect the expressions of miR-143 and pri-miR-143.Expressions of miR-143 and pri-miR-143 in squamous cell carcinoma of cervix were obviously lower than those in normal cervical tissue（P0.05）.Spearman correlation analysis showed that the expression of miR-143 was positively associated with the expression of pri-miR-143（r=0.723,P=0.000）.The level of miR-143 was significantly different in squamous cell carcinoma of cervix with different histological grades（P=0.005）.The expressions of miR-143 in small cell type of squamous cell carcinoma of cervix was significantly lower than that in the other type one.No significant difference was found in miR-143 expression in squamous cell carcinoma of cervix with ages,tumor sizes,general types,and FIGO stages（P0.05）.[Conclusion] Primary transcription reduction is an important reason for down-regulation of miR-143 expression.miR-143 might play an important role in the carcinogensis and progress of squamous cell carcinoma of cervix and may be a novel potential therapeutic target for squamous cell carcinoma of cervix.
出处
《肿瘤学杂志》
CAS
2013年第5期373-377,共5页
Journal of Chinese Oncology
基金
温州市科技计划项目(Y20100255)
