摘要
以籼稻品种密阳 46的幼胚作为试验材料 ,研究了植物激素、蔗糖质量分数、琼脂质量分数、pH值以及继代培养等因素对愈伤组织分化的影响。结果表明 ,MS +KT 3mg/L +NAA 0 .5mg/L是较为合适的分化培养基 ,蔗糖 2 %、琼脂 1%、pH值 6.0以及减少继代培养次数均有助于愈伤组织的分化。
Using immature embryos of indica rice cultivar Miyang 46 as explants, to investigate factors influencing plant regeneration. The results showed that MS + KT 3 mg/L + NAA 0.5 mg/L was the optical differentiation medium and that the medium with 2% sucrose, 1% agar,pH6.0 and decreasing the times of subculture could significantly increase the regeneration frequency of rice callus.
出处
《浙江工业大学学报》
CAS
2000年第3期240-242,共3页
Journal of Zhejiang University of Technology
基金
浙江省自然科学基金! (3982 97)
关键词
籼稻
愈伤组织
植株再生
分化
组织培养
indica rice
Miyang 46
hormone
callus
plant regeneration
