摘要
用组织分离技术从野生红菇 (Russula sp.)子实体中分离得 3个菌株。用随机扩增多态性 DNA(RAPD)对红菇子实体和分离菌株的 DNA多样性进行分析 ,以确定 3个分离菌株与子实体之间的亲缘关系。实验使用 16个随机引物检测 40多个位点 ,计算它们之间的相似性。实验结果表明 3个分离菌株和红菇子实体之间的 DNA相似性非常高 ,红菇子实体和对照菌株 [凤尾菇 (Pleurotus sajor- caju )和金针菇 (Flammulina velutipes (Fr)Sing) ]之间的 DNA相似性差距基本上反映了红菇与对照菌株之间的种属差距。推测
Three strains were obtained from the sporocarp of wild Russula using tissue culture isolation.Random amplified polymorphic DNA(RAPD) was used to approach genetic diversity of wild Russula and three strains, so as to decide the relation between three strains and sporocarp. Over forty genetic sites were checked by using sixteen random primers, and the genetic similarity between three strains and Russula sporecarp was studied.The result showed that they were greatly similar and the similarity gap of DNA between Russula sporocarp and the checked strains showed the interspecies distance. A preliminary conclusion can be drawn from the experiment that the three strains were isolates of Russula.
出处
《广西科学》
CAS
2000年第3期222-224,共3页
Guangxi Sciences
基金
国家自然科学基金! ( 3 945 0 0 5 )
广西自然科学基金!(桂科配 96180 0 5 )
