摘要
目的:了解本院血液真菌感染的菌种分布和耐药情况,为临床用药提供依据。方法:收集2008年1月-2011年12月4年间住院患者的血液标本,经BacT/ALERT 3D血培养仪培养,血琼脂培养基及沙保弱培养基分离培养真菌,用念珠菌显色培养基和ID32C鉴定板条进行菌种鉴定,ATB FUNGUS3微量稀释板测定药敏。结果:14752份血液标本共分离出150株真菌,分离率为1.02%,其中以白假丝酵母菌为主,占39.33%,其次为热带假丝酵母菌,占26.67%。药敏实验发现真菌对氟康唑和伊曲康唑耐药性较高(5.4%和2.7%),对5-氟胞嘧啶和伏立康唑较为敏感(99.3%和97.3%),对两性霉素均为敏感。结论:我院血液真菌感染分离株多数为假丝酵母菌属真菌,对氟康唑和伊曲康唑的抗菌活性下降,建议治疗首选两性霉素和5-氟胞嘧啶。
Objective: To investigate the distribution and drug resistance of fungi in blood and provide the evidence for clinical therapy.Methods: The blood was collected in Lishui hospital between January 2008 and December 2011,cultured with BacT/ALERT 3D.The fungi were isolated with blood agar plate and Sobaurandps agar culture medium,identified with CHROM agar colored medium and ID32C.The susceptibility test was then performed with ATB FUNGUS 3 micro dilution plate.Results: There were 150 fungal isolates of the 14752 blood specimens,and the isolating rate was 1.02%.The prevalence of C.albicans was the main type of the isolates(39.33%),and followed by C.tropicalis(26.67%).Fungi was high resistance to fluconazole and itraconazole,reaching 5.4% and 2.7%,respectively.Flucytosine and voriconazole,reaching 99.3% and 97.3%,respectively.All isolates were sensitive to amphotericin B.Conclusion:In Lishui hospital,the majority of fungi separated from blood were candida species.Antifungi activity of Flucytosine and voriconazole were lower than others,amphotericin B and 5-flucytosine were still recommended as the first choice for fungemia treatment.
出处
《中国卫生检验杂志》
北大核心
2013年第5期1305-1307,共3页
Chinese Journal of Health Laboratory Technology
关键词
血液真菌感染
病原真菌
耐药性
Blood fungal infection
Pathogenic fungi
Drug-resistance