摘要
使用荧光探针FDA标记的HepG2细胞模型及细胞荧光显微图像自动分析法,对川楝子的23个化学组分进行快速筛查,发现5个组分具有明显毒性。对其中的2个组分进行液质联用分析,推测鉴定了10个化学成分,制备并鉴定出其中3个成分的分子结构(meliasenin B,trichilinin D,1-O-tigloy-1-O-debenzoylohchinal)。进一步实验研究发现,这3个成分对HepG2细胞呈量-毒关系,提示川楝子中这些成分可能引起肝毒性。
Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MSn, and 3 of them （meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal） were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第11期1820-1822,共3页
China Journal of Chinese Materia Medica
基金
国家“重大新药创制”科技重大专项(2011ZX09307-002-01)
浙江省科技计划项目(2008C23065)
关键词
川楝子
HEPG2细胞
中药毒性成分
毒性成分快速筛查
肝毒性
Toosendan Fructus
HepG2 cells
toxic compounds in traditional Chinese medicine
fast screening of toxic compounds
hepatotoxicity
