摘要
目的观察高氧肺损伤新生大鼠肺组织中P38-丝裂原活化蛋白激酶(P38)的活化与基质金属蛋白酶-2(MMP-2)mRNA表达水平的变化,并探讨P38的活化对MMP-2 mRNA表达的影响。方法将36只新生Sprague-Dawley大鼠随机分为空气组、高氧组和SB203580干预组,每组12只。建立高氧肺损伤动物模型,处理组给予相应的干预。各组分别于第3天和第7天处死大鼠,常规苏木精-伊红染色,观察肺组织病理学变化;Western blot检测肺组织P38蛋白的活化水平,RT-PCR检测肺组织MMP-2 mRNA表达的变化。结果高氧组大鼠肺组织P38的活化和MMP-2 mRNA表达水平较空气组和干预组明显增强,差异有统计学意义(P<0.01)。结论 P38在新生大鼠高氧肺损伤中活化增强,可能参与MMP-2 mRNA表达的调控。
Objective To observe P38 mitogen-activated protein kinase （P38 MAPK） and matrix metalloproteinase-2 （MMP-2） mRNA expression level changes in neonatal rats with hyperoxia-induced lung injury, and to investigate the influence of P38 MAPK activation on MMP-2 mRNA expression. Methods Thirty-six Sprague-Dawley （SD） rats were randomly divided into three groups： air control, hyperoxia and SB203580-treated hyperoxia （n = 12 ）. The rats were sacrificed on the 3rd and 7th days and the lungs were removed. Hematoxylin-eosine staining was used to observe the pathological changes in lung tissues. Results Compared with the air and SB203580-treated groups, levels of 1938 MAPK and MMP-2 mRNA significantly increased in the hyperoxia group （ P 〈 0.01 ）. Conclusions Expression of P38 MAPK increases in neonatal rats with hyperoxia-induced acute lung injury and this may play a role in control of the expression of MMP-2 mRNA.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2013年第5期383-386,共4页
Chinese Journal of Contemporary Pediatrics
