摘要
目的探讨热处理后体外培养的人表皮黑素细胞和人角质形成细胞（HaCaT细胞）分泌d黑素细胞刺激素（a-MSH）的变化。方法将体外培养的黑素细胞及HaCaT细胞分别给予热处理（42℃，每天60min，连续3d），实时荧光定量PCR法（RT—PCR）及酶联免疫吸附测定法（ELISA）检测两种细胞分泌的a-MSH的变化。结果黑素细胞热处理组（1．5862±0．3262）与对照组（1．0000±0．2715）相比，Ⅱ-MSHmRNA表达均明显增加（P〈0．05）；HaCaT细胞热处理组（1．8013±0．1216）与对照组（1．0000±0．2532）相比，mRNA水平也有明显增加（P〈0．05）。ELISA结果：黑素细胞热处理组（0．3142±0．0469）蛋白表达水平明显高于对照组（0．2557±0．0330，P〈0．05）；HaCaT细胞热处理组（0．4632±0．0345）蛋白水平也明显高于对照组（0．4389±0．0399，P〈0．05）。结论热处理可促进人黑素细胞及HaCaT细胞d—MSH分泌。
Objective To evaluate the in vitro effect of heat treatment on the secretion of a-melanocytestimulating hormone （oL-MSH） by human melanocytes and HaCaT keratinocytes. Methods Cultured normal human melanocytes from forskin tissue and HaCaT cells were treated with hyperthermia at 42 ℃for 1 hour per day for 3 days. Subsequently, real time fluorescence-based quantitative PCR and enzyme-linked immunosorbent assay （ELISA） were performed to detect the mRNA and protein expressions of a-MSH, respectively. Results The mRNA expression level of a-MSH was significantly higher in heat-treated melanocytes and HaCaT cells than in those untreated （1.5862 ±0.3262 vs. 1.0000± 0.2715, P 〈 0.05; 1.8013 ± 0.1216 vs. 1.0000 ± 0.2532, P 〈 0.05）. Moreover, ELISA showed a statistical increase in the supernatant level of a-MSH protein in heat-treated melanoeytes and HaCaT cells compared with those untreated （0.3142± 0.0469 vs. 0.2557 ± 0.0330, P 〈 0.05; 0.4632 ± 0.0345 vs. 0.4389 ± 0.0399, P 〈 0.05）. Conclusion Heat treatment can promote the secretion of a- MSH by melanocytes and HaCaT cells.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2013年第6期401-403,共3页
Chinese Journal of Dermatology
基金
国家自然科学基金（81271745）
