摘要
本文采用反相高效液相色谱建立了长柄扁桃仁系列产品中苦杏仁苷含量的测定方法。长柄扁桃仁系列产品中的苦杏仁苷经甲醇提取,以V(甲醇)∶V(水)∶V(乙腈)=2∶85∶13为流动相进行洗脱,在220nm检测波长下,测得长柄扁桃仁中苦杏仁苷的平均质量分数为1.44%。方法线性范围为50～300μg/mL,线性相关系数r=0.9996,检出限为1.2μg/mL,平均回收率为98.97%(n=5)。
A method of reversed-phase high performance liquid chromatography（RP-HPLC）has been developed for the determination of amygdalin in amygdalus pedunculatus pall products.The sample of amygdalin in amygdalus pedunculatus pall was extracted in methanol.The separation was perfomed with a mobile phase of methanol and water and acetonitrile（2∶85∶13）and a detection wavelength of 220nm.The average content of amygdalin in amygdalus pedunculatus pall detected by this method is 1.44%.The linear range was from 50to 300μg/mL（r=0.9996）.The limit of detection for amygdalin was 1.2μg/ mL,the average recovery was 98.9%（n=5）.The method is accurate,reproducible and can be used for the quality control of amygdalin.
出处
《分析科学学报》
CAS
CSCD
北大核心
2013年第3期367-370,共4页
Journal of Analytical Science
基金
陕西省科技统筹创新工程计划(No.2012KTC203-05
2011KTCL03-04)
陕西省科技厅社发攻关项目(No.2011K16-05-08)
榆林市2010年产学研合作项目(校地合作类)
关键词
长柄扁桃仁
反相高效液相色谱
苦杏仁苷
Amygdalus pedunculatus Pall
Reversed-phase high performance liquid chromatography
Amygdalin
