摘要
从疑似副猪嗜血杆菌病发病猪场采集58份病料,分离到2株革兰氏阴性短小杆菌。对分离菌株进行培养特性、生化特性、血清型分型研究以及PCR检测和测序分析,结果表明,分离的两株细菌均为血清4型副猪嗜血杆菌。对分离菌株进行药敏试验分析,结果显示分离株对头孢噻肟、头孢噻吩、替米考星高度敏感,对四环素、丁胺卡那、恩诺沙星、环丙沙星中度敏感,对甲氧苄氨嘧啶、复方新诺明、链霉素、阿莫西林、新霉素均耐药。
The two small gram - negative bacilli were isolated from 58 samples of pigs with suspected infection by Haemophilus parasuis in Henan. Isolates were identified as Haemophilus parasuis with the culture characteristics, biochemical method, PCR and sequecing. Two isolates were serotyped by kieletein - rapp - gabriedsonas as serotype 4. Results of drug sensitivity test showed that isolates were strong resistance to trimethoprim, sulfamethoxazole, streptomycin, amoxicillin, neomycin, highly sensitive to ceftiofur, cefotaxime, tilmicosin and sensitive to tetracycline, amikacin, enrofloxacin, ciprofloxacin.
出处
《中国兽药杂志》
2013年第6期16-20,共5页
Chinese Journal of Veterinary Drug
关键词
副猪嗜血杆菌
分离
鉴定
血清型
药敏试验
Haemophilus parasuis
isolation
identification
serotypes
drug sensitivity test
