摘要
目的:研究千柏鼻炎片及其有效成分对小鼠过敏性鼻炎模型的治疗作用。方法:BALB/c雄性小鼠在实验第1,7,14天ip 200μL 0.5 g.L-1卵清白蛋白(OVA)以及20 g.L-1氢氧化铝。从第22天起每只小鼠鼻腔滴注50 g.L-1OVA溶液20μL,连续滴注10 d,对照组用生理盐水代替OVA。除对照组外,取造模成功的小鼠50只随机分为5组,其中鼻炎模型组连续7 d鼻腔滴注50 g.L-1OVA溶液10μL;千柏鼻炎片组、绿原酸组和对羟基肉桂酸组在OVA滴注前10 min ig给药,剂量分别17.2 g.kg-1,260,30 mg.kg-1;地塞米松(阳性对照)组在OVA滴注前30 min sc 5 mg.kg-1;对照组则继续用生理盐水滴注。测定各组动物血清免疫球蛋白E(IgE)和组胺的含量,并取小鼠鼻黏膜HE染色病理切片进行观察。结果:千柏鼻炎片、绿原酸和地塞米松明显抑制过敏性鼻炎导致的喷嚏、搔鼻和流清涕等典型症状(P<0.001),并使鼻黏膜充血、水肿和炎性损伤减轻。千柏鼻炎片、绿原酸和地塞米松还能够显著地抑制由过敏性鼻炎引起的血清中IgE和组胺含量的增加(P<0.001)。结论:千柏鼻炎片对过敏性鼻炎有治疗作用,绿原酸可能是千柏鼻炎片的药效活性成分之一。
Objective： To investigate the therapeutic effects of Qianbai Biyan tablet and its main ingredients on allergic rhinitis in mice.Method： BALB/c male mice were administrated with 200 μL ovalbumin（OVA,0.5 g.L-1） and aluminium hydroxide（20 g.L-1） on the first,fourth,seventh day of the experiment.Starting from the 22nd day,each mouse was poured with 20 μL OVA（50 g.L-1） by nasal drip for 10 days consecutively,but physiological saline instead of OVA in control group.Take 50 modeling successful mice to divide into five groups randomly,the allergic rhinitis model group were nasal drop 10 μL OVA solution（50 g.L-1） and Qianbai Biyan tablet group（17.2 g.kg-1.d-1）,chlorogenic acid group（260 mg.kg-1.d-1） and ρ-coumaric acid（30 mg.kg-1.d-1） group were administrated orally in 10 minutes prior to OVA infusion.Meanwhile,the positive drug group were treated with dexamethasone 5 mg.kg-1.d-1 by subcutaneous injections in 30 minutes prior to OVA administration,and the control group were drop with saline continuously.Immunoglobulin E（IgE） and histamine in serum were detected and the nasal mucosa of mice was evaluated by pathological observation.Result： Qianbai Biyan tablet,chlorogenic acid and dexamethasone could obviously inhibit the typical symptoms of allergic rhinitis,including sneezing,scratched nose and thin or watery nasal discharge and so on.Moreover,the nose mucosa membrane hyperemia,dropsy and the inflammatory damage induced by allergic rhinitis were all reduced,and the increase of IgE and histamine level in the serum was decreased.Conclusion： Qianbai Biyan tablet is effective for the treatment of allergic rhinitis,and chlorogenic acid may be the one of active ingredients of Qianbai Biyan tablet.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第12期246-249,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金青年基金(30801544)
教育部新世纪优秀人才计划(NCET-11-1054)
