摘要
目的:将成功构建的人pcDNA3.1-GCNF真核表达载体瞬时转染宫颈癌HeLa细胞,观察GCNF基因对HeLa细胞生物学功能的影响。方法:将重组质粒pcDNA3.1-GCNF转染HeLa细胞,通过MTT比色法、基质胶黏附实验和Transwell侵袭试验对转染后HeLa细胞增殖、黏附和侵袭力进行分析。结果:成功获得瞬时转染的pcDNA3.1-GCNF HeLa细胞,转染目的基因组(0.239±0.014)与转染空质粒组(0.198±0.020)及未转染组(0.204±0.012)相比,细胞增殖最快(P<0.05)。转染目的基因后细胞粘附、侵袭力未见明显改变。结论:GCNF基因促进了体外培养的宫颈癌HeLa细胞增殖,但对HeLa细胞粘附、侵袭力没有明显的影响。
Objective： To transfect cervical cancer HeLa cells with constructed successfully human pcDNA3.1 -GCNF eukaryotic expression vector, and observe the effect of GCNF gene on biological behaviors of HeLa cells. Methods： HeLa cells were transfected with recombinant pcDNA3.1 - GCNF plasmid ; MTT colorimetric method, matrigel adhesion experiment, and Transwell invasive test were used to analyze proliferation, adhesion, and invasion abilities of HeLa cells after transfection. Results： HeLa cells after transient transfection with pcDNA3.1 - GCNF plasmid were obtained successfully, the cell proliferation speeds in pcDNA3.1 - GCNF plasmid transfection group, null vector transfection group, and non - transfection group were （0. 239 ±0. 014） , （0. 198±0. 020） , and （0. 204 ±0. 012） , respectively, the cell proliferation speed in pcDNA3. 1 - GCNF plasmid transfeetion group was the fastest （ P 〈 0. 05 ） ; after transfection, the adhesion and invasion abilities of HeLa cells didnt change significantly. Conclusion： GCNF gene promotes the proliferation of cervical cancer HeLa cells in vitro, but it has no impact on adhesion and invasion abilities of HeLa cells.
出处
《中国妇幼保健》
CAS
北大核心
2013年第17期2777-2781,共5页
Maternal and Child Health Care of China
基金
石河子大学自然科学及技术创新基金项目〔ZRKX2010YB24〕
关键词
GCNF
宫颈癌
转染
粘附
增殖
侵袭
GCNF
Cervical cancer
Transfection
Adhesion
Proliferation
Invasion
