摘要
在用ＭＴＴ测定细胞存活率以研究人表皮癌细胞ＫＢ－３－１ 及其抗阿霉素细胞株ＫＢ－Ａ－１对化疗药物阿霉素、长春花碱和秋水仙碱的多药抗性时，发现培养基中血清浓度的变化对ＭＴＴ法测定结果有影响。吸光值在血清浓度为ｗ ＝ ０．０２ 时最高，ｗ ＝ ０．３０ 时最低，ｗ ＝ ０．０５～０．２０ 之间波动不大。而不加血清使甲酉尤溶解困难而产生误差。培养基中的血清（常为ｗ ＝ ０．１０）不影响ＭＴＴ法的结果，在完全培养基中用ＭＴＴ法测定了两株细胞的抗药性。ＫＢ－Ａ－１ 对阿霉素、长春花碱和秋水仙碱的相对抗药性分别为ＫＢ－３－１ 的１７６、４５ 和１３７ 倍。ＭＴＴ法可简便地用于抗癌药物的筛选。
To determine the relative resistances of KB A 1 cells to the parent cell line KB 3 1,MTT colorimetric assay was used to evaluate the cell growth and survival.In the experiment,It was found that the serum can affect the result of MTT assay,and the optical density reached the maximum with the serum concentration of w =0.02,and the minimum with w =0.30.While at the concentrations between w =0.05~0.20 it fluctuated a little.When no serum was added,the formazan product could not be easily dissolved and the error was inevitably introduced.So,in the ordinary range around w =0.10,the addition of serum to the reaction medium will not cause error to the MTT assay and the experiment can be done easily.This MTT assay was used to perform drug sensitivity assays of KB 3 1 and its drug resistant mutant,KB A 1.The relative resistances of KB A 1 to colchicine,vinblastine and adriamycin are 137,45 and 167 respectively compared with KB 3 1.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第1期103-106,共4页
Journal of East China University of Science and Technology
基金
国家自然科学基金!资助项目(39680032)
国家科委医药技术创新博士项目
关键词
MTT法
多药抗性
肿瘤
化疗药物
MTT
colorimetric assay
serum
cell viability
chemotherapeutical drug
