摘要
目的:研究吉林、兰州地区婴幼儿人星状病毒(HAstV)腹泻流行情况和分子特征。方法:对吉林地区417份和兰州地区290份腹泻标本采用one-step PCR方法检测HAstV,用HAstV基因组ORF1b区部分核苷酸片段初筛,再用ORF2区部分核苷酸序列分型。结果:707份腹泻标本中共检出HAstV阳性17例,总阳性率为2.4%,其中吉林地区和兰州地区分别检出7例和10例。12份HAstV ORF2区阳性的样本均为HAstV 1型,除1株属于Lineage 1a群,其他均属于Lineage 1b群。HAstV主要感染2岁以下婴幼儿,在吉林春季为主要流行季节,而在兰州春季和秋季为主要流行季节。结论:HAstV是引起婴幼儿病毒性腹泻的主要病原之一,HAstV 1型是主要的流行株,2岁以下为感染发病的高危人群,不同地区HAstV流行季节不同。
Objective： To explore the prevalence status and molecular features of astrovirus diarrhea in infants from Jilin and Lanzhou.Methods： The astrovirus expressions in 417 diarrhea specimens from Jinlin and 290 specimens from Lanzhou were detected by one-step PCR.Partial nucleotide fragments in ORF1b region of astrovirus genome were screened,which were somatotypedbg using partial ORF2 sequences.Results： Seventeen postive astrovirus cases in 707 diarrhea specimens,which included 7 cases from Jilin and 10 cases from Lanzhou were detected,the total postive rate was 2.4%.The postive ORF2 specimens in 12 cases were HAstV 1,which belonged to Lineage 1b cluster except 1 Lineage 1a cluster.The HAstV infection mainly distributed in infants less than 2 years,the main morbidity season in Jinlin and Lanzhou were Spring and Autumn,respectively.Conclusions： Astrovirus is the main pathogen of virus diarrhea in infants,HAstV 1 is common serotype,children less than two years old are high-risk group and main morbidity season is difference in different areas.
出处
《蚌埠医学院学报》
CAS
2013年第5期607-609,共3页
Journal of Bengbu Medical College
关键词
人星状病毒
儿童
腹泻
流行病学
human astrovirus
child
diarrhea
epidemiology
