摘要
采用拟反相色谱法以极性硅胶柱配以高水量的流动相,测定奶茶中咖啡因的含量,以0.03 mol·L-1甲酸钠溶液(pH 2.9)-乙腈(95∶5)为流动相,5 min内可快速完成样品测定。其线性范围为0.2～1 000mg·L-1,线性系数(r)为0.999 5,检出限(S/N=3)为0.05 mg·L-1。方法的回收率为98%～103%,相对标准偏差(RSDs)为1.9%～3.6%。
A rapid and sensitive method of pseudo reversed phase liquid chromatography（pseudo-RPLC） was developed for the analysis of caffeine in milk tea.The samples were separated on a bare silica column coupled with aqueous-rich mobile phase（pH 2.9,0.03 mol？L-1sodium formate-acetonitrile,95∶5）,and tested within 5 min.The calibration curve was linear in the concentration range of 0.2-1 000 mg？L-1 with correlation coefficient of 0.999 5.The recoveries at a spiked level of 20 mg？L-1 ranged from 98% to 103% with RSDs 1.9%-3.6%.
出处
《分析测试学报》
CAS
CSCD
北大核心
2013年第6期742-746,共5页
Journal of Instrumental Analysis
关键词
咖啡因
奶茶
快速测定
拟反相色谱法
caffeine
milk tea
rapid determination
pseudo reversed phase liquid chromatography
