摘要
建立了高效液相色谱/光化学反应器/荧光检测器测定茶叶中黄曲霉毒素B1的方法。用乙腈水溶液（V∶V=86∶14）提取黄曲霉毒素B1,提取液经净化柱和黄曲霉毒素B1免疫亲和柱净化,高效液相色谱测定。在黄曲霉毒素B1标准溶液质量浓度为0.591~5.91μg/L时,峰面积与浓度呈现良好的线性关系,黄曲霉毒素B1的回收率为85.4%~98.9%（添加量分别为0.510μg/kg、7.090μg/kg和14.180μg/kg）,相对标准偏差为0.2%~1.8%,方法检出限为0.1μg/kg。运用所建立方法对市售的8个茶样及加标样品中的黄曲霉毒素B1进行检测,结果显示该方法选择性强、灵敏度高,适合茶叶中黄曲霉毒素B1的测定。
A new method for the sensitive determination of Aflatoxins B1 in tea by high performance liquid chromatography with photoelectric reactor and fluorescence detector was established. A solution of V（acetonitrile） ： V（H20）=86 ： 14 was used to extract Aflatoxins B1 from tea. The extracted solution was then purified by a multifunctional and immuneaffinity column, respectively. The peak area and the concentration of Afiatoxins B1 showed a good linear relationship within the range from 0.591 μg/L to 5.91 μg/L with a linear correlation coefficient （r） of 0.9994. The recoveries at the concentrations studied [low level （7.090 μg/kg）, high level （14.180 μg/kg）] were between 85.6% and 98.9% with a relative standard deviations ranging from 1.7% to 1.8%. The limit of detection （LOD） is 0.1 μg/kg （S/N=3）. The limit of quantitation （LOQ） is （0.591 μg/kg）. The new method was used to analyze eight tea samples collected from the local markets and negative results were obtained. The method is suitable for detection of Aflatoxins B 1 in tea with high selectivity and sensitivity.
出处
《茶叶科学》
CAS
CSCD
北大核心
2013年第3期237-241,共5页
Journal of Tea Science
关键词
黄曲霉毒素B1
茶叶
高效液相色谱
多功能净化柱
免疫亲和柱
光化学反应器
Aflatoxins B1, tea, high performance liquid chromatography, multifunctional purifying column, immunoaffinity column, photochemical reactor
