摘要
以蚕豆根尖为材料,采用蚕豆根尖细胞的微核试验和染色体畸变试验方法,研究不同取样点生活污水对蚕豆根尖细胞的致畸效应。结果表明:生活污水能诱发微核率升高,而且在一定范围内,其微核率随污染物浓度升高而增加,但高于一定浓度后有下降趋势;不同取样点的污水均使蚕豆根尖细胞有丝分裂指数下降;生活污水还能诱导蚕豆根尖细胞产生较高频率的染色体畸变。所以生活污水蚕豆根尖细胞具有明显的致畸效应。
The paper investigated the genotoxicity effect of domestic sewage from different treatment processes on the cell of broad bean root tip. The micronucleus and chromosome aberration assay were conducted to determine the micronucleus rate and chromosome aberration rate of broad bean root tip ceils induced by domestic sewage. The result indicated that domestic sewage could increase the micronucleus rate of the sells of broad bean root tip. Within certain concentration range of pollutant, the micronucleus rate increased with the increase of the concentration of pollutants, but beyond certain range, the micronu- cleus rate decreased with further increase of the concentration of pollutants. Domestic sewage could de- crease the cell mitosis index. Furthermore, it also caused the increase of chromosome aberration rate. The conclusion is that domestic sewage has obvious genotoxicity effect on the cell of broad bean root tip.
出处
《水资源与水工程学报》
2013年第3期201-203,共3页
Journal of Water Resources and Water Engineering
关键词
生活污水
蚕豆
微核率
染色体畸变率
domestic sewage
broad bean
micronucleus rate
chromosome aberration rate
