摘要
目的建立高效液相色谱法实时监测患者血浆中甲氨蝶呤浓度,为临床合理用药提供参考。方法以Agilent Eclipse XDB-C18柱(4.6×150mm,5μm)为色谱柱,流动相为醋酸(pH=2.86)-甲醇(82.5:17.5,v/v),流速:0.8ml/min,检测波长306nm,柱温柱温:40℃,样品经高氯酸沉淀蛋白后直接进样。结果表明血浆中甲氨蝶呤的质量0.3～10.20μg/ml内线性关系良好,r=1(n=3),检测限为0.03μg/ml。方法回收率为96.43%～99.49%,提取回收率为:77.04%～85.61%。日内和日间RSD均<5%。结论该方法简便、准确、重复性好,适用于甲氨蝶呤血药浓度监测。
Objective To establish a HPLC method for the determination of the plasma concentration of methotrexate（MTX）.Methods Seperation and determination were achieved on a Agilent Eclipse XDB-C18 column（4.6 × 150 mm,5μm）with the mobile phase consisted of acetic acid（pH = 2.86）methanol（82.5： 17.5）at a flow rate of 0.8 ml/min.The UV detection wavelength was set to 306nm.The column temperature was 40℃ .The plasma samples were treated with perchloric acid for sedimentation.Results The good linearity was from 0.3 to 10.20 μg/ml,r = 1（n = 3）.The minimum detectable concentration for MTX was 0.03 μg/ml.The method recoveries of methotreexate in high,medium and low concentrations were from 96.43% to 99.49% ,and the extraction recoveries were from 77.04% to 85.61% .The within day and between day precision of variation was less than 5% .Conclusion This method is simple,accurate and reliable.It is suitable for the blood drug concentration monitoring of MTX in clinic.
出处
《中国现代药物应用》
2013年第11期7-8,共2页
Chinese Journal of Modern Drug Application
关键词
高效液相色谱法
甲氨蝶呤
血药浓度
HPLC
Methotrexate
Plasma drug concentration monitoring
