摘要
目的:测定不同产地艾叶中桉油精、侧柏酮、樟脑、龙脑4个挥发性成分的含量。方法:采用气相色谱程序升温法测定。使用安捷伦HP-5毛细管柱(30 m×0.32 mm×0.25μm),载气为高纯氮气(99.99%),流速0.6 mL.min-1,分流比为10∶1,进样口温度240℃,进样量1μL,检测器温度250℃,程序升温(起始温度50℃,以4.0℃.min-1升至75℃,保持10 min,以1.0℃.min-1升至100℃,以8.0℃.min-1升至150℃,保持2 min,以3.0℃.min-1升至165℃,保持5 min,以8.0℃.min-1升至250℃,保持3 min)。结果:以该方法测定桉油精、侧柏酮、樟脑、龙脑的回收率在95%～103%之间。各产地艾叶样品均含有桉油精、樟脑、龙脑,部分样品含有侧柏酮。结论:该方法可为艾叶质量标准的提高提供科学数据。
Objective：To determine the contents of four volatile components（ eucalyptol,thujone, camphor and bor-neol） of Artemisia argyi from different origins. Method：The essential oil of Artemisia argyi from different origins was extracted by steam distillation, and quantitatively determined by GC linked with Agilent HP-5 capillary column（ 30 m×0.32 mm ×0. 25 μm）. The carrier gas was high purity nitrogen at a flow rate of 0.6 mL · min^-1. The inlet tem-perature was 240 ℃, the injection volume was 1.0 p,L, and the FID detector temperature was 250 ℃. The column temperature was raised by program：the initial temperature was 50 ℃, raised to 75 ℃ with a rate of 4 ℃ ·min^-1, maintained for 10 min,then raised to 100 ℃ with a rate of 1 ℃ · min^-1 ,raised to 150 ℃ with a rate of 8 ℃ · min^-1, maintained for 2 min, again raised to 165 ℃ with 3 ℃ · min^-1, maintained 5 min, and the last raised to 250 ℃ with the rate of 8 ℃ ·min^-1, maintained 3 min. Results： The recoveries of eucalyptol, thujone, camphor and borneol were between 95% - 103%. All samples contained eucalyptol, camphor and borneol, and some of them con-tained thujone. Conclusion： The method can contribute to the improvement of Artemisia argyi quality standard by providing scientific data.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第6期1033-1036,共4页
Chinese Journal of Pharmaceutical Analysis
基金
国家重点基础研究发展计划(973计划)项目(2009CB522906)
关键词
艾叶
桉油精
侧柏酮
挥发性成分含量测定
龙脑
樟脑
质量标准提高
中药多成分分析
气相色谱
Artemisia argyi
eucalyptol
thujone
volatile components assay
borneol
camphor
improvement of quali-ty standard
multi - components analysis of traditional Chinese medicine
GC
