摘要
酸菜是我们日常生活中经常食用的食物。实验从东北自然发酵酸菜中分离纯化出1个菌株,参照《伯杰氏细菌鉴定手册》,进行形态学初步鉴定,并通过16SrRNA序列分析,以序列同源性为基础构建相关属种的系统进化树。结果表明:分离株与Lactobacillus plantarum strain H2位于同一个分支中,且与其的同源性达到97%,因此最终鉴定该分离株helsc为植物乳杆菌。
The sauerkraut was the common food in our daily lives. The bacteria was isolated and purified from the Northeast naturally fermented sauerkraut.The strain was identified preliminary by morphology reference according to ＂Taxonomic outline of the prokaryotes Bogey＇s manual of systematic bacteriology＂, and the method of 16S rRNA sequence homology analysis was used to construct a phylogenetic tree of genus and species. The results showed that, the isolated stain and Lactobacillus plantarum strain H2 were located in the same branch and the sequence homology was 97%. Therefore, the isolated strain was identified as Lactobacillus plantarum finally.
出处
《黑龙江八一农垦大学学报》
2013年第3期63-66,共4页
journal of heilongjiang bayi agricultural university
关键词
酸菜
东北
自然发酵
植物乳杆菌
鉴定
sauerkraut
northeast
wild fermentation
lactobacillus plantarum
identification
