期刊文献+

乌司他丁对创伤性脑损伤的保护作用研究 被引量:8

Protective effects of ulinastatin on mice with traumatic brain injury
下载PDF
导出
摘要 目的探讨乌司他丁(UTI)对创伤性脑损伤的保护作用和具体机制。方法 BALB/c小鼠27只,随机分为空白组(shame组)、生理盐水对照组(control组)、损伤组(TBI组)和乌司他丁预处理组(UTI组)。利用小鼠神经功能评分(NSS)法判断乌司他丁的治疗作用;Western blot方法检测脑组织中cathepsin B蛋白表达变化情况。结果与shame组比较,TBI组的cathepsin B蛋白表达明显增加(P<0.05),UTI组cathepsin B蛋白表达显著减少(P<0.05);与空白组比较,UTI组能明显减轻神经功能障碍,各组间差异有统计学意义(P<0.05)。结论乌司他丁预处理可能通过调控cathepsin B减轻细胞死亡,从而有效改善脑损伤后神经功能,具有显著的神经保护作用。 Objective To investigate the potential therapeutic effect and the related mechanism of ulinastatin (UTI) on mice with traunmtic brain injury. Methods A total of 27 BALB/c mice were randomly divided into four groups :sham group, TBI group, physiological saline control group and the UTI pretreatment group (UTI group). Neurological functions of mice were treasured by nerve function score (NSS) ; Western blot was applied to detect the expression of cathepsin B protein of brain tissues. Results There was significant difference in the expression of cathepsin B among sham group, TBI group and UT/group (P 〈0. 05). The expression of cathepsin B protein was decreased obviously in UTI group (P 〈0.05). Conclusion The UTI pretreatment may reduce the cell death by regu- lating cathepsin B protein, which thereby can effectively improve the neurological functions following TBI.
出处 《中华神经外科疾病研究杂志》 CAS 2013年第3期244-246,共3页 Chinese Journal of Neurosurgical Disease Research
基金 天普研究基金资助项目(01200908)
关键词 乌司他丁 脑损伤 组织蛋白酶B Ulinastatin Traumatic brain injury Cathepsin B
  • 相关文献

参考文献3

二级参考文献32

  • 1刘辉,姚咏明,董月青,于燕,盛志勇.高迁移率族蛋白B1诱导巨噬细胞Janus激酶/信号转导及转录激活子通路活化的研究[J].中国危重病急救医学,2004,16(10):592-595. 被引量:10
  • 2孙宝贵,汪玮.慢性心力衰竭治疗中血管紧张素转换酶抑制剂的应用[J].中国实用内科杂志,2005,25(7):582-584. 被引量:55
  • 3谢康,黄跃生,安瑞,周军利,张家平.乌司他丁对严重烧伤患者伤后早期心肌损害的防治作用[J].中华烧伤杂志,2006,22(3):180-183. 被引量:32
  • 4Dhainaut JF,Cariou A,Laurent I.Myocardial dysfunction in sepsis.Sepsis,2000,4:89-97.
  • 5Parrillo JE,Parker MM,Natanson C,et al.Septic shock in humans:advances in the understanding of pathogenesis,cardiovascular dysfunction,and therapy.Ann Intern Med,1990,113:227-242.
  • 6Court O,Kumar A,Parrillo JE,et al.Clinical review:myocardial depression in sepsis and septic shock.Crit Care,2002,6:500-508.
  • 7Kisseleva T,Bhattacharya S,Braunstein J,et al.Signaling through the JAK/STAT pathway,recent advances and future challenges.Gnen,2002,285:1-24.
  • 8Cooney RN.Suppressors of cytokine signaling (SOCS):inhibitors of the JAK/STAT pathway.Shock,2002,17:83-90.
  • 9Legendre F,Dudhia J,Pujol JP,et al.JAK/STAT but not ERK1/ERK2 pathway mediates interleukin (IL)-6/soluble IL-6R down-regulation of type Ⅱ collagen,aggrecan core,and link protein transcription in articular chondrocytes.J Biol Chem,2003,278:2903-2912.
  • 10Aosasa S,Ono S,Mochizuki H,et al.Mechanism of the inhibitory effect of protease inhibitor on tumor necrosis factor alpha production of monocytes.Shock,2001,15:101-105.

共引文献23

同被引文献61

引证文献8

二级引证文献62

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部