摘要
目的:探讨宫颈鳞癌组织miR-34a下调的表达机制。方法:应用茎环RT Realtime-PCR方法检测48例宫颈鳞癌和20例正常宫颈组织中miR-34a的表达;通过甲基化特异PCR检测miR-34a启动子甲基化情况,分析5-Aza-dC去甲基化处理的Caski宫颈癌细胞miR-34a表达变化,观察miR-34a启动子甲基化对miR-34a失调表达的影响。结果:miR-34a在宫颈鳞癌组织中的表达明显低于正常宫颈组织,差异有统计学意义(P<0.05)。甲基化特异PCR检测显示,宫颈鳞癌组织miR-34a启动子区甲基化比率明显高于正常宫颈组织(P<0.05)。5-Aza-dC去甲基化处理人类乳头状瘤病毒(HPV)16阳性的Caski细胞后,miR-34a表达明显升高(P<0.05)。结论:除HPV16 E6外,启动子甲基化也是抑癌基因miR-34a在宫颈鳞癌组织中异常表达的原因。
Objective： To analyze the mechanism of miR-34a down-expression in squamous cell carcinoma of cervix. Methods： The expression of miR-34a in 48 samples of squamous cell carcinoma of cervix and 20 samples of normal cervical tissue were analyzed by stem-loop RT Realtime-PCR. Promoter methylation of miR-34a was detected by methylation-specific polymerase chain reaction （MSP）. The change of miR-34a expression was evaluated after 5-AzadC hypomethylation treatment and the influence of miR-34 expression was discussed with the miR-34a promoter methylation. Results： Expression of miR-34a in the squamous cell carcinoma of cervix was obviously lower than that in the normal cervical tissue （P〈0.05）. MSP data showed that the methylation ratio of miR-34a promoter in cervical cancer was significantly higher than that in normal cervical tissue （P〈0.05）. After 5- AzadC hypomethylation treatment, miR-34a expression in Caski cell increased （P〈0.05）. Conclusion： Except for HPV16 E6, promoter methylation is also the mechanism of miR-34a down-expression in the tissues of squamous cell carcinoma of cervix.
出处
《温州医学院学报》
CAS
2013年第5期311-314,共4页
Journal of Wenzhou Medical College
基金
温州市科技局科研基金资助项目(Y20100255)
