摘要
研制了一种用于体外评价胶基型无烟气烟草制品烟碱释放行为的模拟咀嚼机,并利用该设备结合高效液相色谱仪测试了4种市售胶基型无烟气烟草制品的体外烟碱释放率。该设备主要包括溶媒存储瓶、蠕动泵、恒温槽、循环水箱、载物台、空气压缩机、气缸、电机、气动夹子和释放液收集装置。其中循环水箱和载物台形成的测试腔用来模拟人体口腔,以人工唾液为溶媒,空气压缩机带动气动夹子反复挤压被测样品以模拟人体咀嚼状态。体内、体外烟碱释放测试结果表明:利用该模拟咀嚼机评价胶基型无烟气烟草制品烟碱释放行为的方法简便可靠,测试结果与人体使用该类产品时的烟碱释放情况接近。该技术对胶基型无烟气烟草制品的质量控制、烟碱生物利用度评价以及烟碱调控具有一定的应用价值。
A simulated chewing machine was developed for evaluating in vitro the release behavior of nicotine from gum-based smokeless tobacco products, and the in vitro nicotine release rates from four kinds of gum-based tobacco products on the market were tested by combining that machine with HPLC. The chewing machine was mainly composed of a container for solvent, two peristaltic pumps, a thermostat, two circulating water tanks, a sample stage, an air compressor, a cylinder, a motor, a pneumatic clamp and a liquid collector. The test chamber formed by the circulation tanks and sample stage simulated a buccal cavity, which ensured nicotine release at a constant temperature of 37 ℃ into artificial saliva, the pneumatic clamp pushed by the air compressor compressed the test sample repeatedly simulating the chewing action. The in vivo and in vitro nicotine release tests showed that evaluating the release behavior of nicotine from gum-based tobacco products with the simulated chewing machine was simple and reliable, the test results were close to the nicotine release from gum-based tobacco products taken by a human being. The technique was of practical uses for the quality control of gum-based tobacco products, the evaluation of nicotine bioavailability as well as the control of nicotine.
出处
《烟草科技》
EI
CAS
北大核心
2013年第6期31-36,共6页
Tobacco Science & Technology
基金
国家烟草专卖局重点项目"无烟气烟草制品研制含化型无烟气烟草制品"(412008AA0620)
关键词
胶基型无烟气烟草制品
烟碱
体内摄取率
体外释放
模拟咀嚼机
Gum-based smokeless tobacco product
Nicotine
In vivo ingestion
In vitro release
Simulated chewing machine
