摘要
目的:研究miR-215在结肠直肠癌(colorectal cancer,CRC)组织中的表达及其临床意义。方法:研究对象是我院2007年1月至6月的进展期CRC病人,从入选病人的石蜡包埋组织中提取microRNA,进行实时定量PCR检测。结果:共有42例CRC病人入选,男25例,女17例;直肠癌18例,结肠癌24例。miR-215在CRC组织中的表达水平低于配对的癌旁正常组织(P<0.001);CRC组织中miR-215下降不明显者的生存时间较长(P=0.035)、P53表达水平较高(r=0.556,P<0.001)。结论:miR-215可能成为CRC的预后标志物。
Objective To investigate the expression and clinical significance of miR-215 in colorectal cancer （CRC）. Methods The data of patients with advanced colorectal cancer from Jan to Jun 2007 in our hospital were analyzed, microRNA was extracted from formalin-fixed paraffin-embedded tissue and the levels of miR-215 were quantified by quantitative real-time polymerase chain reaction. Results Forty-two cases with CRC were enrolled （25 males and 17 females）. Among them, 18 cases had rectal cancer and 24 colon cancer. The levels of miR-215 expression were lower in CRC tissues than in adjacent non-tumor tissues （P〈0.001）. Patients with slight-decrease of miR-215 levels in CRC tissue had longer survival time （P=0.035） and higher levels of P53 expression （r=0.556, P〈0.001）. Conclusions miR-215 might be a prognostic biomarker for colorectal cancer.
出处
《外科理论与实践》
2013年第3期247-251,共5页
Journal of Surgery Concepts & Practice
基金
上海市闵行区科学技术委员会项目(2012MHZ059)
上海市科学技术委员会项目(10411968500)
