摘要
目的:探讨survivin基因沉默对人肝癌耐药株阿霉素化疗敏感性的影响。方法:针对人survivin基因设计并合成3条siRNA序列,分别用脂质体法转染人肝癌BEL-7402细胞(3组)。以未转染的BEL-7402细胞为对照,分别用RT-PCR法,免疫细胞化学技术及MTT法检测各组细胞survivin mRNA与蛋白的表达,及对阿霉素敏感性。结果:与对照组比较,各转染组细胞survivin mRNA与蛋白的表达均明显降低(均P<0.05);对照组细胞对阿霉素的IC50值为(20.60±2.86)μg/mL,各转染组细胞对阿霉素的IC50值分别为(11.53±1.46)μg/mL,(14.13±1.82)μg/mL,(15.53±0.46)μg/mL,与对照组比较差异均有统计学意义(均P<0.05)。结论:抑制survivin基因的表达能增加BEL-7402细胞阿霉素化疗敏感性。
Objective： To investigate the influence of survivin gene silencing on chemotherapy sensitivity of drug resistant cell line of human hepatocellular carcinoma to doxorubicin. Methods： Three siRNA sequences targeting human survivin gene were designed and synthesized, and then were transfected into hepatocellular carcinoma BEL-7402 cells （3 groups of cells） by liposome-mediated method, respectively. Using the untransfected BEL-7402 cells as control, the expressions of survivin gene and protein as well as sensitivity to doxorubicin in each group of cells were determined by RT-PCR method, immunocytochemical staining and MTT assay, respectively.Results： Compared with control group of cells, both survivin gene and protein expressions in each transfected group of cells were significantly decreased （all P〈0.05）. The value of half inhibitory concentration （IC50） of doxorubicin for control group of cells was （20.60±2.86） μg/mL, while the IC50 values for the three groups of transfected cells were （11.53±1.46）μg/mL, （14.13±1.82） μg/mL and （15.53±0.46） μg/mL respectively, all of which had statistical differences versus control （all P〈0.05）. Conclnsion： Inhibition of survivin gene expression can increase the chemotherapy sensitivity of BEL-7402 cells to doxorubicin.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2013年第6期742-746,共5页
China Journal of General Surgery
基金
广东省卫生厅医学科研基金资助项目(B2008145)
广东医学院科研基金资助项目(2008)
湛江市科技攻关资助项目(2012C3102025)
关键词
癌
肝细胞
RNA干扰
抗药性
肿瘤
存活素
Carcinoma, Hepatocellular
RNA Interference
Drug Resistance, Neoplasm
Survivin
