摘要
利用穴盘扦插育苗,研究了5种质量分数的盐(NaCl)胁迫处理对8个柳树无性系幼苗(SH13,SH16,SH19,SH23,SH24,SH30,172,2460)形态特性的影响,测定了叶片SOD活性、POD活性、MDA含量的变化情况。结果表明:柳树扦插苗成活率与生物量之间中度正相关(相关系数r=0.55),显示二者之间存在不紧密的关联。MDA含量与SOD活性、POD活性显著正相关(相关系数分别为rSOD=0.745,rPOD=0.794),说明抗氧化酶活性与叶片MDA含量紧密关联。柳树扦插苗对盐胁迫的耐受点在0.4%～0.6%之间,0.8%的NaCl胁迫对柳树幼苗造成不可逆伤害。SH30、SH19扦插扩繁相对容易,生长迅速,抗盐碱能力理论上较强,值得进一步研究。
The effects on morphological character of eight willow clones seedling （SH13, SH16, SH19, SH23, SH24, SH30, 172, 2460） treated with different NaC1 concentrations have been researched systematically. The SOD activity, POD ac- tivity and MDA content in leaves were determined. The results show that the correlation between survival ratio of Willow cut- ting seedlings and biomass are moderate positive correlation （correlation coefficient r= 0.55）, it means that they are not closely related. The correlation between MDA content and SOD activity, POD activity are significantly positive correlation （correlation coefficient rsoD =0. 745, rpOD =0. 794）, it shows that the activity of antioxidant enzyme and MDA content in leaves are closely related to each other. The salt stress tolerance of Willow cutting seedlings is from 0.4% to 0. 6%. More than 0. 80/oo NaC1 stress cause irreversible damage to the willow seedlings. SH30 and SH19 cutting seedlings are easy on mass breeding, and also having strong salt stress tolerance, so they are worthy for further research.
出处
《山东林业科技》
2013年第3期9-12,共4页
Journal of Shandong Forestry Science and Technology
基金
林业公益性行业科研专项"我国北方野生柳树资源评价及开发利用研究"(201304115)
山东省农业良种工程重大课题"林木种质资源收集保护与评价"(鲁农良字[2010]6号)
2005年山东省农业良种工程课题"柳树的良种选育"
关键词
柳树无性系
盐胁迫
抗氧化酶
Willow clone, Salt stress , antioxidant enzymes
