摘要
目的探讨丝裂霉素C对兔角膜碱烧伤后角膜新生血管(CNV)形成及角膜血管内皮生长因子(VEGF)的影响。方法将66只健康家兔随机选取6只,为A组(正常组),余60只家兔行左眼眼角膜碱烧伤,建立角膜新生血管动物模型,将其随机平分为B组(实验对照组)和C组(实验干预组)。分别于1,4,7,14,21d显微镜下观察各组CNV的生长情况,计算新生血管面积,并采用免疫组化法检测家兔角膜组织中VEGF的表达情况。结果 A组没有CNV生成,C组和B组相比CNV的生长明显受到了抑制;免疫组织化学染色结果示:A组VEGF微弱表达,B组和C组的表达明显高于A组,C组VEGF的表达低于B组,且差异具有统计学意义(P<0.05)。结论丝裂霉素C可有效抑制角膜碱烧伤后CNV的生成及角膜组织中VEGF的表达。
Objective To explore the effects of mitomyein C on corneal neovaseularization and vascular en- dothelial growth factor after alkali burn. Methods Six of sixty-six healthy rabbits were randomly selected as group A （normal group） ,and tile model of corneal neovascularization were established by alkali burn of the left corneas of the other sixty rabbits which were randomly divided into group B （ experimental control group） and group C （ experimental in- tervention group）. On the 1 st,4th ,7th and 14th ,21st day,the growth of CNV were observed while the size of new vessels were figured with the slit lamp,and then the expression of VEGF in the rabbit corneas was detected with immunohisto- chemistry method. Results Except group A,both group B and group C showed the growth of CNV,but the growth of CNV in group C was obviously inhibited compared with that in group B ;meanwhile,the result of the immunohistoehe,nis- try method showed group A had low expression of VEGF while the expression of VEGF in group B and C was obviously higher than that of group A, but the expression of VEGF in group C was much lower than that in group B,the difference was statistically significant（ P 〈 0.05 ）. Conclusion Mitomyein C can effectively inhibit the growth of CNV and the ex- pression of VEGF in the cornea after alkali burn.
出处
《潍坊医学院学报》
2013年第3期197-199,共3页
Acta Academiae Medicinae Weifang
