摘要
测定了根田鼠在室内驯化 2周后褐色脂肪组织 ( BAT)的蛋白含量、线粒体蛋白含量和细胞色素 C氧化酶活性的变化。动物进行如下 4种处理 :2 3℃ ,1 6L∶ 8D;2 3℃ ,8L∶ 1 6D;5℃ ,1 6L∶ 8D和 5℃ ,8L∶ 1 6D。结果表明 ,短光照可刺激根田鼠的 BAT线粒体细胞色素C氧化酶的活性增加 ;低温驯化可导致根田鼠的 BAT线粒体蛋白含量增加 ;短光照加低温可进一步促进酶的活性增加。结合个体水平的产热特点 ,说明根田鼠在产热能力的季节驯化过程中 ,光周期是一主要的季节调节信号 ,环境温度进一步增强光照的作用 ,环境温度和光周期共同作用以诱导其产热能力等方面的生理调节。研究结果支持了环境温度和光周期共同作用以调节动物产热变化的学说。
The effects of ambient temperature and photoperiod on the thermogenic characteristics of brown adipose tissue(BAT) in root voles (Microtus oecnomus ) were examined. Root voles were acclimated to the following conditions for two weeks: 1) long day warm temperature (16L∶8D, 23 ℃); 2) Long day cold temperature (16L∶8D, 5 ℃); 3) short day warm temperature (8L∶16D, 23 ℃); and 4) short day cold temperature (8L∶16D, 5 ℃). The results showed that both temperature and photoperiod were the important environmental factors for variations of thermogenesis in root voles. Low temperature and/or short photoperiod could induce increases in BAT protein contents, BAT mitochondrial protein contents, and cytochrome C oxidase activity of BAT mitochondria. Combined with the responses of thermogenesis to these factors at organism level, it seemed that root voles were more sensitive to short photoperiod, and cold temperature could strengthen this effect. The results support the hypothesis that ambient temperature combined with photoperiod induce the thermogenic adjustments for small mammals.
出处
《兽类学报》
CAS
CSCD
北大核心
2000年第2期123-129,共7页
Acta Theriologica Sinica
基金
国家自然科学基金资助项目!( 3 94 70 1 2 1 )
关键词
根田鼠
褐色脂肪组织
驯化
产热能力
光照
温度
Root vole( Microtus oeconomus )
Brown adipose tissue(BAT)
Acclimation
Mitochondria
