摘要
目的观察保健灸法对自然衰老大鼠血脂含量及脑组织中Caspase-3、Bcl-2表达的影响,探讨保健灸法调控血脂及细胞凋亡的作用。方法 SD雌性大鼠42只,其中3月龄14只,为青年组,12月龄28只,随机分为对照组和保健灸组。保健灸疗后采用ELISA法检测各组大鼠血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量;采用免疫组化法检测脑组织中Caspase-3、Bcl-2蛋白的表达水平。结果与对照组比较,保健灸组血清中TC、TG、LDL-C含量下降,HDL-C含量升高,脑组织Caspase-3蛋白表达水平明显下降(P<0.05),脑组织中Bcl-2蛋白表达水平明显升高(P<0.05)。结论保健灸法可有效调节随着增龄而导致的高血脂状态;可以抑制由起始因子Caspase-3引发的细胞凋亡,并通过上调Bcl-2蛋白的表达而起到延缓衰老的作用。
Objective Observe the effect of health-care moxibustion on blood lipid contents and Caspase-3,Bcl-2 protein expression of senile rats to discuss the effect of health-care moxibustion on regulation of blood lipid and apoptosis.Methods 42 SD female rats,of which there are 14 rats in youth group,28 rats in 12 moons＇ age group.Randomly divide them into control group and health-care moxibustion group.After two months of experiment,test the content of the rat serum total cholesterol（TC）,triglycerides（TG）,low density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C） content and the expression level of caspase-3,Bcl-2 protein.Results Compared with 12 moons＇ age rats of control group,the content of serum TC,TG,LDL-C down,HDL-C content rise and expression level of Caspase-3 protein in brain tissue significantly decreased（P 0.05）,Bcl-2 protein increased（P 0.05）.Conclusion Health-care moxibustion can effectively reduce blood lipid content,regulate lipid metabolism and restrain apoptosis by regulating Caspase-3,Bcl-2 protein expression to delay senescence.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2013年第6期1537-1539,共3页
Lishizhen Medicine and Materia Medica Research
基金
辽宁省自然基金资助项目(No.20092039)
辽宁省教育厅资助项目(No.2008S146)
