摘要
以栽培品种"银峰80天"的下胚轴与子叶为外植体,建立花椰菜高效组织再生体系,并研究卡那霉素浓度、侵染时间和共培养时间等因素对花椰菜愈伤组织分化的影响。结果表明:以MS+TDZ 0.2 mg/L+NAA0.1mg/L作为分化培养基时,下胚轴的分化频率达88.9%,子叶的分化频率达97.6%;农杆菌介导花椰菜遗传转化时,OD600=0.4,下胚轴和子叶的侵染时间为1 min,下胚轴和子叶分别共培养1 d和3 d后进行筛选,有利于花椰菜再生芽的分化获得抗性苗。
Using hypocotyls and cotyledons of broccoli cultivars ＂yinfeng 80 days＂ as the explants, an efficient regeneration system of cauliflower were established, and the factors influencing transformation efficiency, including kanamycin concentration, infection duration, as well as co-cultivation time etc, were optimized in the present work. The results showed that the shoot formation from hypocotyls and cotyledon might reach to 88.9% and 97.6% , respectively MS medium supplemented with 0.2 mg/L TDZ and 0.1 mg/L NAA gave the best effect for tissue differentiation. The hypocotyls and cotyledons were infected with Agrobacterium for 1 min, subsequently co-cultured for 1 d to hypocotyls or 3 d to cotyledons, by which Kan ^＋ resistant shoots were regenerated.
出处
《山地农业生物学报》
2013年第3期189-193,共5页
Journal of Mountain Agriculture and Biology
基金
国家转基因新品种培育重大专项"安全转基因"子项目(2011ZX08010-003-2)
关键词
花椰菜
植株再生
遗传转化
cauliflowers
plant regeneration
genetic transformation
