摘要
目的采用反相高效液相色谱法（HPLC）分析蔚蓄中杨梅苷和槲皮苷的含量。方法以AgilentLC-C18（250mm×4．6mm，5μm）色谱柱，以乙腈．0．1％磷酸溶液（18：82）作为流动相，流速为1．0ml/min，柱温：30℃；检测波长：256nm。结果杨梅苷和槲皮苷分别在1．22-24．30gg／ml和0．77-15．40pg／ml范围内有良好的线性关系，平均加样回收率分别为98．9％、99．6％，RSD分别为1．11％、1．09％。结论本方法可用于蔚蓄中杨梅苷和槲皮苷的定量分析。
Objective To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Polygonum aviculare L. Methods The Agilent LC-Cls （250 mm×4.6 mm, 5 μm） column was used, the mobile phase consisted of acetonitrile： 0.1% H3PO4 （18 82） , the flow rate was 1.0 ml/min, the column temperature was 30℃ the detecting wavelength was at 256 nm. Results The calibration curve was linear within a range of 1.22-24.30 μg/ml and 0.77-15.40μg/ml, the average recovery of this method was 98.9%, 99.6% and the RSD was 1.11%, 1.09%, for the myricetrin and quercitroside respectively. Conclusion The method is simple, repeatable and accurate. It can be applied in quantitative determination of myricetrin and quercitroside in Polygonum aviculare L..
出处
《国际中医中药杂志》
2013年第7期613-615,共3页
International Journal of Traditional Chinese Medicine
关键词
萹蓄
杨梅苷
槲皮苷
高效液相色谱法
Polygonum aviculare L.
Myricetrin
Quercitroside
RP-HPLC
