摘要
目的:探讨大豆皂甙对糖尿病大鼠血、尿及肾脏IL-6的影响。方法:以Wistar雄性大鼠为研究对象,将实验动物分为三组即对照组、糖尿病模型组和大豆皂甙给药组,三组动物分别每日给予生理盐水、生理盐水、大豆皂甙(SS,10 mg/kg)灌胃3个月,动物处死前一天用代谢笼收集24小时尿液,然后第二天处死取血液及肾组织。采用ELISA法检测血、尿和肾脏IL-6的蛋白水平;采用实时荧光定量PCR法检测肾脏IL-6 mRNA水平;采用HE染色和免疫组织化学染色检测肾组织的病理改变及IL-6的表达。结果:模型组大鼠血、尿和肾脏IL-6的蛋白水平、mRNA水平及免疫组化阳性强度明显高于对照组和SS给药组(P<0.01),SS给药组略高于对照组但无统计学意义(P>0.01);HE染色显示正常组大鼠肾脏结构清晰,肾小球、肾小管形状规则;SS给药组大鼠肾脏结构清晰,肾小球、肾小管形状较为规则;模型组大鼠肾组织结构不清晰,肾小球、肾小管形状较规则。结论:大豆皂甙能降低糖尿病大鼠血、尿和肾脏IL-6的水平,提示大豆皂甙对糖尿病大鼠的肾脏有一定的保护作用,IL-6可能参与了糖尿病肾病的发病及病理变化过程。
Objective： To explore the effect of soyasaponins on IL-6 of blood, urine and kidney of diabetic rats. Methods： Wistar male rats, the experimental animal were randomly divided into three groups, control group, diabetic model group and SS treat- ment group, the rats in three groups were given daily saline, daily saline, SS（ 10 mg/kg） orally three month, the day before the animal were killed with metabolic cages to collect 24 h urine , and then the next day were sacrificed blood and kidney tissue. ELISA assay blood, urine and kidneys of IL-6 protein levels; using real-time PCR method to detect IL-6 mRNA levels of kidney; HE staining and immunohistocbemieal staining detected renal tissue pathological changes and the expression of IL-6. Results： The model rats blood, u- rine and kidney protein levels of IL-6, mRNA levels and immunohistochemical staining intensity was significantly higher than that in the control group and the SS administration group（ P 〈 0.01 ）, SS administration group was slightly higher than in the control group, but no statistically significant（ P 〉 0.01 ） ; HE staining showed a clear structure of nomal rat kidney glomerular and tubular shape rules; clear of the SS dose group rat kidney glomerular and tubular shape more rules ; model of kidney tissue structure was not clear glomerular and tubular shape irregular. Conclusion： Soybean saponin to reduce diabetic rats blood , urine and kidney of IL-6 levels, suggesting that soy saponins a protective effect on the kidneys of diabetic rats, IL-6 may be involved in the pathogenesis of diabetic nephropathy and pathological changes of the process.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第6期593-595,599,共4页
Chinese Journal of Immunology
基金
吉林省教育厅(2012460)基金项目资助
