摘要
目的建立养血益肾胶囊的质量标准。方法采用薄层色谱法对天麻、淫羊藿、丹参进行定性鉴别。采用高效液相色谱-蒸发光散射检测器法对黄芪甲苷含量进行测定,Diamonsil C18(2)色谱柱(4.6 mm×250 mm,5μm),乙腈-水(33∶67)为流动相等度洗脱,流速1.0 mL/min,柱温35℃,检测器为蒸发光散射检测器,漂移管温度105℃,空气流速2.7 L/min。结果薄层色谱法得到的结果斑点清晰,重复性好。黄芪甲苷在进样量0.397 0～5.955 0μg范围内峰面积的自然对数与进样量的自然对数线性关系良好,r=0.999 9,平均加样回收率为102.0%,RSD=1.5%(n=6)。结论本方法简便快捷,适于该制剂的质量控制。
Objective To establish the quality standard for Yangxue Yishen Capsules.Methods Rhizoma Gastrodiae,Herba Epimedii and Radix et Rhizoma Salviae Miltiorrhizae in the formula were identified qualitatively by TLC.The content of Astragaloside IV was determined by HPLC-ELSD method.The Diamonsil C18（2） column（4.6 mm×250 mm,5 μm） was used.The mobile phase was acetonitrile-water（33∶67） with 1.0 mL/min flow rate at 35 ℃.The temperature of drift tube for ELSD was 105 ℃,the air flow rate was 2.7 L/min.Results The TLC displayed clear spots on their right positions repeatedly.The linear range of Astragaloside IV was 0.397 0-5.955 0 μg（r =0.999 9）.The average recovery was 102.0%,RSD was 1.5%（n =6）.Conclusion The method is simple and quick.It can be used as quality control method of Yangxue Yishen Capsules.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第7期52-54,共3页
Chinese Journal of Information on Traditional Chinese Medicine
