摘要
目的建立毓麟茶的质量控制方法。方法采用薄层色谱法对毓麟茶中黄芪、蛇床子、五味子、枸杞子进行定性鉴别,采用高效液相色谱法测定君药五味子有效成分五味子醇甲的含量。色谱条件:Welch Materials C18色谱柱(250 mm×4.6 mm,5μm),甲醇-水(70∶30)为流动相,检测波长250 nm。结果薄层色谱鉴别方法简便,专属性强。五味子醇甲0.222～0.2220μg范围内呈良好的线性关系,r=0.9999,平均加样回收率为98.96%,RSD=0.74%。结论所建立的方法可用于该制剂的质量控制。
Objective To establish the quality standard of Yuling Tea for its quality control.Methods Astragalus,Fructus cnidii L,Schisandra chinensis and Lycii Fructus in Yuling Tea were identified by TLC.The content of Schisandrin was determined by HPLC.Welch Materials C18 column（250 mm×4.6 mm,5 μm） with mobile phase of methanol-water（70∶30） was used.The detective wavelength was 250 nm.Results The TLC for identification was simple and special.Schisandrin showed a good linear relationship in 0.222-0.222 0 μg,r =0.999 9.The average recovery was 98.96%,and RSD was 0.74%.Conclusion The method can accurately determine the content of Schisandrin in Yuling Tea.It can be used for quality control of the preparation.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第7期61-63,共3页
Chinese Journal of Information on Traditional Chinese Medicine
