摘要
目的建立测定妇阴舒洗液中绿原酸含量的HLPC方法。方法采用高效液相色谱法,选用Hypersil ODSC18(250 mm×4.6 mm 5μm)色谱柱;流动相为甲醇-0.5%醋酸酐水溶液(20∶80);柱温:25℃,检测波长为327nm,流速为1.0 mL/min。结果绿原酸在0.25～2.50μg范围内与峰面积呈良好线性关系(r=0.9999),平均回收率98.28%,RSD=0.80%(n=5)。结论该方法操作简便、灵敏度高、重现性好、测定结果可靠,可用于妇阴舒洗液中绿原酸的含量测定。
Objective To establish the determination method for the content of chlorogenic acid in FuY- inShu lotions by HPLC. Methods The content of chlorogenic acid was determined by HPLC with Dia- monsil column （4.6mm × 250mm, 5μm） and column temperature of 25℃. The mobile phase was a mixture of methyl alcohol and 0.5% acetic anhydride （20： 80） with the flow rate of 1.0 mL/min and the detection wavelength of 327 nm. Results The content of chlorogenic acid presented a good linearity from 0.25 μg to 2.50 ug （ r = 0. 9999）. The average recovery was 98.28% and RSD was 0.80% （ n = 5 ）. Conclusion This method is accurate and sensitive with a good reproducibility and could be used for the determination of chlorogenic acid content in FuYinShu lotions.
出处
《遵义医学院学报》
2013年第3期264-266,共3页
Journal of Zunyi Medical University
