摘要
目的:探讨桑菊清解汤对急性吸烟大鼠氧化与抗氧化系统的影响。方法:将50只雄性大鼠,按体重随机分为正常对照组、模型组、NAC(乙酰半胱氨酸颗粒)组及高、低剂量桑菊清解汤组,每组10只。正常对照组、模型组灌胃蒸馏水;高、低剂量组桑菊清解汤灌胃相应剂量桑菊清解汤,均按1mL/100g体重给药;NAC组将NAC以蒸馏水溶解,配制成浓度为0.2g/mL的溶液,按200mg/kg体重灌胃给药。除正常对照组外,其他各组每天空腹灌胃1次后进行被动吸烟造模,连续7天。第7天后取血浆及肺泡灌洗液(BALF),检测还原型谷胱甘肽(GSH)含量及测定血清超氧化物歧化酶(SOD)活性。结果:与正常对照组比较,模型组BALF、血浆中GSH含量及BALF和血清中SOD活性均低于正常对照组(P<0.01);与模型组比较,低剂量桑菊清解汤组BALF中GSH含量及BALF和血清中SOD活性升高(P<0.05),而高剂量桑菊清解汤组和NAC组BALF、血浆中GSH含量及BALF和血清中SOD活性均升高(P<0.01)。高、低剂量桑菊清解汤对急性被动吸烟损伤动物模型气道有一定保护作用且其作用呈一定的剂量依赖关系。结论:桑菊清解汤对被动吸烟所致大鼠急性肺损伤有一定的保护作用,且其保护作用可能与抑制自由基的产生、减少脂质过氧化有关。
Objective： To investigate the effects of San^u Qin^ie Decoction（SQD） on oxidative and anti-oxidative system of acute smoking rats. Methods： Fifty male rats were equally randomized into 5 groups, and they were normal control group, model group, n-acetyl cysteine（NAC） group, and low-dose and high dose SQD groups. The normal control group and model group were given distilled water intragastrically, low-dose and high-dose SQD groups were intragastrically given low- or high-dose SQD lmL/100g body weight, and NAC group was given 0.2g/mL NAC 200mg/kg body weight. Rats except the normal control group were given smoking exposure daily after intragastrical administration for 7 continuous days. After 7 days, reduced glutathione hormone（GSH） content and superoxide dismutase（SOD） activity in the plasma and bronchoalveolar lavage fluid（BALF） were detected. Results： Compared with the normal control group, GSH content and SOD activity in BALF and plasma were decreased in the model group（P 〈0.01）. Compared with the model group, GSH content in BALF and SOD activity in BALF and plasma were decreased in low-dose SQD group（P 〈 0.05）, and GSH content and SOD activity in BALF and plasma were all decreased in high-dose SQD group and NAC group（P〈 0.01）, indicating that SQD had dose-dependent protective effect on the airway of rats with acute pulmonary injury induced by passive smoking. Conclusion： SQD can protect the acute smoking rats from lung injury, and the protective mechanism may be related with the inhibition of the generation of free radicals and with the decrease of lipid peroxidation.
出处
《新中医》
CAS
2013年第7期165-167,共3页
New Chinese Medicine
