期刊文献+

麻醉方法对大鼠生理、血气及血液流变学的影响 被引量:5

The influence of different anesthesia on the physiology,blood gas and hemorheology of rat
原文传递
导出
摘要 目的比较不同麻醉方式对大鼠生理、血气及血液流变学的影响,完善血液代用品标准评价模型的制备。方法 Wistar大鼠(16只)随机分为2组,分别采用戊巴比妥钠腹腔注射和乌拉坦肌肉注射的方法麻醉。记录麻醉起效时间,每隔5 min记录各项生理指标,直至术后40 min,随后抽血检测血气、血细胞及各项流变学参数。结果戊巴比妥钠组和乌拉坦组麻醉起效时间分别约为3.67和31 min,戊巴比妥钠腹腔注射起效显著快于乌拉坦肌肉注射(P<0.01);相对于戊巴比妥钠组,乌拉坦组降低平均动脉压(5、15、30、35、40 min)、收缩压(35、40min)、舒张压(15、30、35、40 min)、脉压差(40 min)和体温(除5 min时间点外),均为P<0.05;术后40 min,乌拉坦组Hct高于戊巴比妥钠组(P<0.05),pH明显降低(P<0.01),血浆粘度降低(P<0.05)。结论乌拉坦肌肉注射麻醉影响大鼠生理、血气分析和血液流变学指标,在进行药效评价时需慎重选择。 Objective To compare the influence of different anesthesia on parameters of physiology,blood gas and hemorheology,and standardize the model used for evaluating the blood substitutes.Methods A total of 16 Wistar rats were divided into 2 groups randomly according to the anesthesia,sodium pentobarbital intraperitoneal injection(S group) or urethane intramuscular injection(U group).The acting time was recorded and the physiological parameters were also evaluated every 5 min till 40 min after operation.Then blood was withdrawn to assess the blood gas,blood cells and hemorrheology parameters.Results The times needed for S group and U group were 3.67 min and 31 min respectively,showing that the former came into effect much faster than the latter(P 0.01).Compared with S group,the U group decreased the mean arterial pressure(5,15,30,35 and 40 min,P 0.05),the systolic pressure(35 and 40 min,P 0.05),the diastolic blood pressure(15,30 and 35 min,P 0.05),the pulse pressure(40 min,P 0.05) and the body temperature(expect for 5 min,P 0.05) significantly.And 40 min after the operation,there were significant higher haematocrit(P 0.05),lower arterial pH value(P 0.01) and plasma viscosity(P 0.05) in U group than those in S group.Conclusion The anesthesia via urethane intramuscular injection should be chosen cautiously because of its influences on rats' physiology,blood gas and hemorrheology parameters.
出处 《中国输血杂志》 CAS CSCD 北大核心 2013年第5期417-420,共4页 Chinese Journal of Blood Transfusion
基金 国家自然科学基金面上基金项目(31271001) 国家高技术研究发展计划主题项目(2010AA021902) 北京市科委首都市民健康项目培育(Z121100005312014)
关键词 麻醉 动物模型 平均动脉压 血液流变学 anesthesia animal model mean arterial pressure hemorheology
  • 相关文献

参考文献10

二级参考文献17

  • 1张清林,王爱平,宋欣,黄春倩,刘秀华,王治乔.乌拉坦诱发小鼠肺肿瘤短期试验研究[J].中国公共卫生学报,1994,13(4):239-242. 被引量:8
  • 2和占龙,鲁帅尧,陈丽雄,王俊斌,赵远,唐永明.速眠新在实验猕猴麻醉中的应用[J].实验动物与比较医学,2005,25(4):236-237. 被引量:7
  • 3高年发,宝菊花.氨基甲酸乙酯的研究进展[J].中国酿造,2006,25(9):1-4. 被引量:87
  • 4龚泰芳,方彩云,陈文,王平年.海藻酸盐的理化特性及其在组织工程研究和临床中的应用[J].中国组织工程研究与临床康复,2007,11(18):3613-3616. 被引量:13
  • 5杨藻宸.医用药理学[M].第3版.北京:人民卫生出版社,1998.
  • 6谢荣.麻醉学(第三版)[M].北京:科学出版社,1994:601,76~78 1999-10-13收稿
  • 7Martini J,Carpentier B,Chávez Negrete A,et al.Beneficial effects due to increasing blood and plasma viscosity.Clin Hemorheol Microcirc,2006,35(1-2):51-57.
  • 8Cabrales P,Intaglietta M,Tsai AG.Increase plasma viscosity sustains microcirculation after resuscitation from hemorrhagic shock and continuous bleeding.Shock,2005,23(6):549-555.
  • 9Tsai AG,Acero C,Nance PR,et al.Elevated plasma viscosity in extreme hemodilution increases perivascular nitric oxide concentration and microvascular perfusion.Am J Physiol Heart Circ Physiol,2005,288(4):1 730-1 739.
  • 10Cabrales P,Tsai AG,Intaglietta M.Hyperosmotic-hyperoncotic versus hyperosmotic-hyperviscous:small volume resuscitation in hemorrhagic shock.Shock,2004,22(5):431-437.

共引文献31

同被引文献82

引证文献5

二级引证文献14

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部