摘要
目的:观察抗柯萨奇B病毒性心肌炎胶囊(KCoxBJN)总皂苷(TS)体外抗柯萨奇B3病毒(CVB3)及对细胞活性的影响。方法:分离培养大鼠原代心肌细胞,以KCoxBJ总皂苷(KCoxBJN-TS)为干预因素,用MTT法测定其对正常心肌细胞的TC50和TC0;观察其对心肌细胞感染CVB372h的搏动频率、细胞病变程度和存活率;测定各组间TCID50及心肌细胞培养上清液中乳酸脱氢酶(LDH)、肌酸激酶(CK)的活性及肿瘤坏死因子-α(TNF-α)含量的变化,判定其细胞活性。结果:KCoxBJN-TS对正常心肌细胞的TC0为6.25mg/mL,TC50为8.89mg/mL,浓度在12.5、25mg/mL时利巴韦林组(R组)心肌细胞存活率高于各中药组,浓度在100mg/mL时两组细胞存活率大致相同,且此两种药物对抑制心肌细胞的活性作用呈剂量依赖性;感染CVB3后心肌细胞的搏动频率明显减慢,细胞病变(CPE)明显,中药组与R组比较均有显著性差异(P<0.01),CPE程度较轻,其中中药高剂量组(KCoxBJN-TS-H组)效果最佳;心肌细胞病毒滴度测定显示,KCoxBJN-TS-H、中药中剂量组(KCoxBJN-TS-M组)与R组有效,而中药低剂量组(KCoxBJN-TS-L组)无明显效果;KCoxBJN-TS-H组能使LDH、CK的释放较CVB3组显著降低(P<0.01);KCoxBJN-TS还可抑制被病毒感染的心肌细胞TNF-α的分泌,KCoxBJN-TS-H组和R组效果较好。结论:KCoxBJN-TS在一定的浓度范围内可抑制CVB3在细胞内的复制,对心肌细胞起到一定的保护作用。
Objective： To observe the effects of anti-CVB myocarditis capsule compound total saponins in vitro CVB3 and the cell activity.Methods： Separation and cultivation of rats＇ myocardial cells,taking KCoxBJN-TS as intervention factors,with MTT method to determine the normal heart muscle cells TC50 and TC0;Observation of the KCoxBJN-TS affecting myocardial cells CVB3 72h infection the throbbing in frequency,cell pathological damage,and survival rate;The TCID50 determination between myocardial cell culture and clear liquid on the lactate dehydrogenase（LDH）,creatine kinase（CK） activity and tumor necrosis factor-α（TNF-α） content changes was determined to judge its cell activity.Results： KCoxBJN-TS on the normal heart muscle cells TC0 is 6.25mg/mL;TC50 is 8.89mg/mL;when the concentration is in 12.5mg/mL,25mg/mL,the survival rate of ribavirin myocardial cells（R） is higher than the TCM groups;the concentration in 100mg/mL,the survival rate of both groups of cells is roughly same;and both medicine can control the myocardial cell activity on dose dependent;after CVB3 infected,the beating frequency of the heart muscle cells is obvious slow,cell（CPE） changes obviously;there are difference between R and TCM group（P〈0.01）,CPE is at a lesser degree,and the KCoxBJN-TS-group H has the best effect,and there is no difference compared with normal group;Myocardial cell virus drops for testing showed that KCoxBJN-TS dose group of high,medium and ribavirin group are effective,while KCoxBJN-TS low dose group has no obvious effect;compared with CVB3 group,the release of LDH,CK significantly reduced in KCoxBJN-TS-H group（P〈0.01）;KCoxBJN-TS-L can inhibit the infection myocardial cell TNF-α secretion,KCoxBJN-TS-H and R effect are much better,and there is no difference compared with normal group.Conclusion： KCoxBJN-TS in certain concentration range can inhibit the replication of CVB3 inside the cell,which play a certain protective effect to myocardial cells.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第7期2104-2108,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家"十一五"规划科技重大专项重大新药创制候选药物课题(No.2009zx09103-442)~~
关键词
抗柯萨奇B病毒性心肌炎胶囊
中药提取物
总皂苷
柯萨奇B3病毒
Anti-CVB myocarditis capsule
Traditional Chinese medicine extracts
Total saponins
The B3 coxsackie virus
