摘要
目的探讨组蛋白去乙酰化酶抑制剂丁酸钠(NaB)对子宫内膜癌细胞株HEC-2B增殖及N-myc下游调节基因1(NDRG1)蛋白表达的影响。方法采用MTT比色法检测不同浓度的NaB处理HEC-2B细胞48、72h后细胞的生长情况;荧光倒置显微镜下观察NaB处理48h后细胞的形态改变;Western blotting法检测NaB处理48h后细胞中NDRG1蛋白的表达情况。结果 NaB能够抑制HEC-2B细胞的增殖,并呈浓度和时间依赖性(P<0.05)。不同浓度NaB作用48h后,荧光倒置显微镜下HEC-2B细胞出现细胞凋亡的特征性改变。随着NaB浓度的增加,HEC-2B细胞中NDRG1蛋白的表达水平逐渐下调。结论 NaB能够抑制子宫内膜癌HEC-2B细胞的增殖,可能与下调NDRG1蛋白的表达有关。
Objective To investigate the anti-proliferative effects induced by sodium butyrate （NaB） on human endometrial carcinoma HEC-2B cell line and its influence on N-myc downstream regulated gene 1 （ NDRG1 ） expression. Methods The HEC-2B cells were exposed to various concentrations of NaB for 48 and 72h. The cell proliferation was evaluated by MTT assay. The morpholog- ical changes of the HEC-2B cells were observed by inverted fluorescence microscope. The expression of NDRG1 protein was determined by Western blotting method. Results MTI＂ assay showed that NaB inhibited the growth of HEC-2B cell line in a dose- and time-de- pendent manner in vitro （ P 〈 0. 5 ）. After NaB treatment for 48h, evident morphological changes with pathognomonic feature of apoptosis were observed. Western blotting showed that the expression of NDRG1 protein were gradually down-regulated with the increasing of NaB concentration. Conclusion NaB can significantly inhibit the growth of human endometrial carcinoma in vitro and its mechanism may be related to the down-regulation of NDRG1 protein expression.
出处
《临床肿瘤学杂志》
CAS
2013年第6期490-493,共4页
Chinese Clinical Oncology
基金
黑龙江省教育厅科学技术研究项目(12511343)1
