摘要
为研究新的种质资源三明显性核不育水稻在转导水稻抗稻瘿蚊基因Gm6过程中的应用效果,以抗蚊青占为Gm6基因供体亲本,三明显性核不育水稻为载体,采用连续回交结合MAS方法把Gm6基因导入受体亲本元丰B中。结果表明:通过对BC5F1代可育株进行筛选,获得含有纯合Gm6基因株系BSC9246,其指纹图谱与元丰B一致,农艺性状与元丰B相似;以元丰A为细胞质供体,经连续的回交转育育成三系不育系BSC9247,杂种优势与元丰A基本一致。利用该方法转导水稻抗病虫基因可以免去雄,确保每一个杂交世代杂交种子的真实性,该方法高效、易行。
Transduction of the gall midge resistant gene, Gin6, for rice using the new germplasm resource of Sanming dominant genic male sterile （SMDGMS） rice was studied. Kanwenqingzhan as the donor and SMDGMS rice as the vector, Gin6 was introduced into the rice three-line maintainer line, Yuanfeng B, by successive backcrosses. The result indicated that the pure lines of BSC9246 with Gin6 gene similar to Yuanfeng B in fingerprint and agronomic traits had been selected from the fertile plants of BCs F1. By using Yuanfeng A as the cytoplasmic donor, the three male sterile lines, BSC9247, were bred through successive backcrosses with the heterosis basically identical to Yuanfeng A. This appeared to be an efficient facile method that could not only eliminate the need of the removal of male plants, but also ensure the purity of each generation of the hybrid seeds.
出处
《福建农业学报》
CAS
2013年第5期438-442,共5页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金项目(2011J01393)
关键词
三明显性核不育
载体
转导
Gm6
： Sanming dominant genie male sterile
vector
transduet
Grn6
