摘要
A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution, the papain-silica composite formed rapidly and oanain was encansulated. The encansulation efficiency and the recovery activity were 82.60% and 83.09%, re-spectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additaonal silica-precipitating species, this papaln encapsulation process, a biomimetic approach, realized high encapsulation efficiency by its autosilification activity under mild conditions （near-neutral pH and ambient temperature）. Fur-thermore, the encapsulated papain exhibits enhanced thermal, pH, recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.
一个新奇方法通过 papain 导致的一个 biomimetic silicification 过程为 papain 固定被开发。由在一个硅石先锋答案孵化 papain， papain 硅石合成很快形成了， papain 被包含。封装效率和恢复活动分别地是 82.60% 和 83.09% 。与面对另外的猛抛硅石的种类在 biosilica 矩阵使不能调动的酶和 biomolecules 相比，这个 papain 封装过程，一条 biomimetic 途径，由它在温和条件(在中立附近的 pH 和周围的温度) 下面的 autosilification 活动的认识到的高封装效率。而且，包含的 papain 展览提高了热， pH，再循环并且存储稳定性。运动分析证明 biomimetic 硅石矩阵显著地没妨碍底层的集体运输或产品的版本。
基金
Supported by the National Natural Science Foundation of China （21006020, 21276060, 21276062）, the Natural Science Foundation of Hebei Province （B2010000035, B2011202095）, the Science and Technology Research Key Project of Higher School in Hebei Province （ZD2010118）, the Application Basic Research Plan Key Basic Research Project of Hebei Province （11965150D） and Open Funding Project of ~e National Key Laboratory ofBiochemi＇cal Engineering （China）.
