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EB病毒阳性肺癌组织中LMP-1、p53、Bcl-2及MMP-9表达 被引量:3

Expressions of LMP-1,p53,Bcl-2 and MMP-9 in Tissues of Lung Cancer with Epstein-Barr Virus Infection
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摘要 [目的]检测EB病毒(EBV)阳性肺癌组织中EBV潜伏感染膜蛋白-1(LMP-1)、p53、Bcl-2及基质金属蛋白酶-9(MMP-9)表达情况,分析它们与肺癌发生发展的关系。[方法]采用原位杂交法检测108例肺癌组织和22例癌旁正常肺组织中EBV编码的小RNA-1(EBV encoded small RNA-1,EBER-1)。免疫组织化学的方法检测EBER-1阳性和阴性肺癌组织中LMP-1、p53、Bcl-2及MMP-9的表达。[结果]108例肺癌组织中EBER-1阳性36例,阳性率33.3%;22例癌旁正常肺组织中EBER-1阳性1例,阳性率4.5%,差异有统计学意义(P<0.01)。EBER-1阳性和阴性的肺癌组织中LMP-1阳性率分别为11.1%和4.2%,差异无统计学意义(P>0.05);在EBER-1阳性肺癌组织中p53、Bcl-2的平均面积(AA)和积分光密度(IOD)均高于阴性组,差异有统计学意义。在EBER-1阳性肺癌组织中MMP-9AA和IOD均高于阴性组,但差异无统计学意义(P>0.05)。[结论]EBV感染可能通过影响LMP-1、Bcl-2、p53和MMP-9在肺癌组织中的表达,进而在肺癌的发生、发展和转移中发挥作用。 Purpose To investigate the expressions of Epstein-Barr virus (EBV) latent infection membrane protein 1(LMP-1),p53,Bcl-2,matrix matalloproteinases-9(MMP-9) in tissues of lung cancer with EBV infection,and to explore their relationship with carcinogenesis of lung cancer. Methods EBV encoded small RNA-1 (EBER-1) in 108 tissue samples of lung cancer and 22 cases of cancer adjacent tissues were detected by in situ hybridization. The expressions of LMP-1,p53,Bcl-2 and MMP-9 in EBV positive and negative cases were detected immunohistochemically. Results Positive expression of EBER-1 was found in 36 of 108 cases with lung cancer (33.3%), and in 1 of 22 cases of cancer adjacent tissues(4.5%),with significant difference(P0.01). Positive rate of LMP-1 in EBER-1 positive lung cancer tissues and EBER-1 negative tissues was 11.1% and 4.2% respectively,without significant difference. Average area (AA) and integral optical density(IOD) of p53 and Bcl-2 in EBER-1 positive cases were higher than those in negative tissues,with significant difference. AA and IOD of MMP-9 in EBER-1 positive tissues were higher than those in negative tissues,without significant difference(P0.05). Conclusion EBV might play a role in carcinogenesis and metastasis of lung cancer by influencing the expressions of LMP-1,p53,Bcl-2 and MMP-9.
出处 《肿瘤学杂志》 CAS 2013年第6期431-434,共4页 Journal of Chinese Oncology
基金 河北省自然科学基金资助项目(H2013209180) 唐山市科技计划项目(121302136)
关键词 肺肿瘤 EB病毒小RNA(EBER-1) 原位杂交 潜伏感染膜蛋白-1(LMP-1) p53 Bcl-2 基质金属蛋白酶-9(MMP-9) lung neoplasms EBV encoded small RNA-1(EBER-1) in situ hybridization latent infection membrane protein 1(LMP-1) p53 Bcl-2 matrix matalloproteinases-9(MMP-9)
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