摘要
目的研究丝氨酸蛋白酶抑制剂(mammary serpin,MASPIN)在卵巢癌的发展和血管生成中的作用。方法采用免疫组化LsAB法对31例正常卵巢组织、35例上皮性卵巢癌(epithelial ovarian cancer,EOC)标本进行MASPIN、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达检测,分析它们与卵巢癌临床病理特点的关系;MASPIN真核表达质粒体外转染卵巢癌SKOV3细胞,用细胞免疫化学及半定量逆转录聚合酶链反应(RT-PCR)方法检测转染后卵巢癌细胞中VEGF的蛋白质和mRNA表达变化。结果 MASPIN、VEGF在卵巢癌中的表达与正常卵巢组织相比明显上调(P<0.05);MASPIN蛋白表达与非浆液性卵巢癌、低临床分期、无腹水形成差异有统计学意义((P<0.05);VEGF蛋白表达与卵巢癌腹水形成差异有统计学意义(P<0.05);卵巢癌中MASPIN蛋白表达与VEGF呈负相关(r=-0.738,P<0.05);细胞免疫组化及半定量逆转录聚合酶链反应(RT-PCR)方法均证实转染MASPIN cDNA的SKOV3细胞中VEGF的表达明显弱于转染空载体组和未转染组(P<0.05),而后两者之间差异无统计学意义(P>0.05)。结论 MASPIN在卵巢癌的发展过程中可能仍发挥着肿瘤抑制基因的作用。MASPIN能从蛋白质和mRNA水平下调卵巢癌中VEGF的表达,并可能通过这一机制抑制卵巢癌血管生成。
Objective To investigate the role of MASPIN in ovarian tumor development and vascularization. Methods The gene expressions of MASPIN and VEGF were detected by immunohistoehemistry in formalin-fixed, paraffin-embedded specimens of normal ovaries（ n = 31 ）and epithelial ovarian cancers （n = 35）. Correlations of expressions of MASPIN and VEGF with clinicopathological parameters of ovarian tumors were statistically analyzed. The recombinant plasmid MASPIN was stable transfeeted into o- varian cancer cell line SKOV3. Changes of VEGF and heparanase at mRNA and protein levels were determined by immunocytochemis- try and RT-PCR, respectively. Results The expressions of MASPIN and VEGF proteins were up-regulated in ovarian cancer cells compared to normal ovaries（P 〈 0. 05）. A significant association was observed in MASPIN protein expression and clinical parameters such as non-serous ovarian carcinoma,lower tumor stage and no ascitic formation（ P 〈 0. 05 ）. VEGF protein expression correlated with ascitie formation positively（ P 〈 0. 05 ）. MASPIN protein expression correlated negatively with VEGF（ r =-0. 738, P 〈 0. 05 ）. The re- sults of both immunocytochemistry and RT-PCR demonstrated that VEGF expression level in SKOV3-maspin cells was lower than that in SKOV3 and SKOV3-vector cells, although the difference was no statistically siguifieant. Conclusion MASPIN might have inhibitory effects on invasion and metastasis of epithelian ovarian cancer, and inhibit the angiogenesis in ovarian carcinoma by down-regulating VEGF expression.
出处
《实用医院临床杂志》
2013年第4期42-47,共6页
Practical Journal of Clinical Medicine
基金
四川省卫生厅科研基金资助项目(编号:070206)
