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Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum 被引量:3

Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum
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摘要 Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. We isolated and characterized the fpBeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus Fusarium proliferatum LF061. Targeted inactivation of the F. proliferatum genomic copy of fpBeas abolished the production of beauvericin. Comparative sequence analysis of the FpBEAS showed 74% similarity with the BbBEAS that synthesizes the cyclic trimeric ester beauvericin in Beauveria bassiana, which assembles N-methyl-dipeptidol monomer intermediates by the programmed iterative use of the nonribosomal peptide synthetase modules. Differences between the organization of the beauvericin loci in F. proliferaturm and B. bassiana revealed the mechanism for high production of beauvericin in F. proliferatum. Our work provides new insights into beauvericin biosynthesis, and may lead to beauvericin overproduction and creation of new analogs via synthetic biology approaches. Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. We isolated and characterized the fpBeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus Fusarium proliferatum LF061. Targeted inactivation of the F. proliferatum genomic copy of fpBeas abolished the production of beauvericin. Comparative sequence analysis of the FpBEAS showed 74% similarity with the BbBEAS that synthesizes the cyclic trimeric ester beauvericin in Beauveria bassiana, which assembles N-methyl-dipeptidol monomer intermediates by the programmed iterative use of the nonribosomal peptide synthetase modules. Differences between the organization of the beauvericin loci in F. proliferaturm and B. bassiana revealed the mechanism for high production of beauvericin in F. proliferatum. Our work provides new insights into beauvericin biosynthesis, and may lead to beauvericin overproduction and creation of new analogs via synthetic biology approaches.
出处 《Science China(Life Sciences)》 SCIE CAS 2013年第7期628-637,共10页 中国科学(生命科学英文版)
基金 supported by the National Basic Research Program of China (2013CB734000, 2012CB725200) the CAS Pillar Program (XDA04074000) Ministry of Science and Technology of China (2011ZX11102-011-11) ZHANG LiXin is an Awardee for the National Distinguished Young Scholar Program in China
关键词 BEAUVERICIN Fusarium proliferatum biosynthesis gene cluster synthetic biology 球孢白僵菌 生物合成 基因簇 枯萎 鉴定 克隆 细胞毒活性 环状三聚体
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  • 1Zhang L, Yan K, Zhang Y, et al. High-throughput synergy screening identifies microbial metabolites as combination agents for the treatment of fungal infections. Proc Natl Acad Sci USA, 2007, 104: 4606-4611.
  • 2Hoffman H L, Ernst E J, Klepser M E. Novel triazole antifungal agents. Exp Opin Invest Drugs, 2000, 9:593-605.
  • 3Hamill R L, Higgens C E, Boaz H E, et al. The structure of beauvericin, a new depsipeptide antibiotic toxic to Artemia salina. Tetrahedron Lett, 1969, 49:4255-4258.
  • 4Roeske R W, Isaac S, Steinrau L, et al. Synthesis and ion-transport properties of peptide antibiotic beauvericin. Fed Proc, 1971, 30: 1282-1288.
  • 5Schwarzer D, Finking R, Marahiel M A. Nonribosomal peptides: from genes to products. Nat Prod Rep, 2003, 20:275-287.
  • 6Finking R, Marahiel M A. Biosynthesis of nonribosomal peptides. Ann Rev Microbiol, 2004, 58:453-488.
  • 7Kopp F, Marahiel M A. Macrocyclization strategies in polyketide and nonribosomal peptide biosynthesis. Nat Prod Rep, 2007, 24:735-749.
  • 8Pieper R, Haese A, Schroder W, et al. Arrangement of catalytic sites in the multifunctional enzyme enniatin synthetase. Eur J Biochem, 1995, 230:119-126.
  • 9Haese A, Schubert M, Herrmann M, et al. Molecular characterization of the enniatin synthetase gene encoding a multifunctional enzyme catalyzing n-methyldepsipeptide formation in fusarium-scirpi. Mol Microbiol, 1993, 7:905-914.
  • 10Peeters H, Zocher R, Kleinkauf H. Synthesis of beauvericin by a multifunctional enzyme. J Antibiotics, 1988, 41:352-359.

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