摘要
为探究NO对山葡萄耐盐性影响的生理机制,以1年生双丰山葡萄扦插苗为试材,采用营养液栽培方法,研究了外源NO供体硝普钠(SNP)处理对50 mmol.L-1NaCl胁迫下山葡萄叶片叶绿素含量、叶绿素荧光参数和抗氧化酶活性的影响。结果表明,外源NO能显著提升盐胁迫下山葡萄叶片超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性,减少丙二醛(MDA)的产生和积累,保护光合机构免受活性氧伤害,从而提高叶片叶绿素含量;另一方面,外源NO使盐胁迫下山葡萄叶片的PSⅡ最大光化学效率(Fv/Fm)、PSⅡ实际光化学效率(ΦPSⅡ)、光化学淬灭系数(qP)和保护性热耗散(ΦNPQ)升高,而初始荧光(Fo)和非调节性能量耗散(ΦNO)显著下降,表明外源NO通过保护性热耗散机制缓解盐胁迫引起的光抑制,增强光合电子传递效率,进而维持PSⅡ的正常功能,最终提高了山葡萄耐盐性。
To evaluate the role of nitric oxide（NO） in plant salt tolerance,this study was conducted in nutrient solution to investigate the effects of exogenous sodium nitroprusside（SNP）,an NO donor,on chlorophyll content,chlorophyll fluorescence parameters and antioxidant enzymes activity in Vitis amurensis ＇Shuangfeng＇ 1-year cutting seedling under 50 mmol·L-1 NaCl stress.The results showed that exogenous NO significantly promoted the activity of superoxide dismutase（SOD） and ascorbate peroxidase（APX） in Vitis amurensis leavs,which led to the decrease of malondialdehyde（MDA）,thus protected chloroplast memberance stability and increased chlorophyll content.On the other hand,exogenous NO increased photochemical efficiency of photosystem II（Fv/Fm）,actual photochemical efficiency（ΦPSII）,photochemical quenching coefficient（qP） and regulated thermal energy dissipation（ΦNPQ） under NaCl stress.However,the minimal fluorescence（Fo） and decreased photo damage（ΦNO） in Vitis amurensis leaves were decreased.These results indicated that NO enhanced the tolerance of Vitis amurensis seedlings to NaCl stress through the protecting mechanism of thermal dissipation which alleviated photo inhibition caused by salt stress,improving photochemical electron transport efficiency and maintaining the normal function of PSII to some extent.
出处
《核农学报》
CAS
CSCD
北大核心
2013年第6期867-872,共6页
Journal of Nuclear Agricultural Sciences
基金
现代农业产业技术体系建设专项资金(农业部
财政部nycytx-30-01)
山葡萄种质资源收集
编目与利用(农业部NB2011-2130135-37)
关键词
山葡萄
盐胁迫
一氧化氮
叶绿素荧光
抗氧化酶
Amur grape
Salt stress
Nitric oxide
Chlorophyll fluorescence
Antioxidant enzymes
