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硫酸盐还原菌生长条件优化及驯化试验研究 被引量:7

The domestication and optimization of sulfate reducting bacteria growth conditions
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摘要 为获得耐Cu2+和耐低pH的硫酸盐还原菌,本研究通过富集培养分离到硫酸盐还原菌,以SO42-为考察指标,优化了其生长因子碳源、pH、COD/SO42-比值和温度,结果表明:乳酸钠作为碳源、pH=7、COD/SO42-=1.6、温度30℃为该菌最优生长条件.通过驯化获得能够在Cu2+浓度为90 mg/L、pH=4.5的条件下生长的硫酸盐还原菌,可为硫酸盐还原菌处理含Cu2+酸性矿山废水工艺提供菌株. In this study, sulfate reducting bacteria was separated by the reducting bacteria with the resistance to Cu2+ and low pH. By taking enrichment culture to obtain sulfate 2- SO4 as the investigating index, growth 2- factors of carbon source, pH, COD/SO4 ratio and temperature were optimized. The resuhs show that these 2- optimal growth conditions for sulfate reducing bacteria are: sodium lactate as a carbon source, pH=7, COD/SO4 =1.6, and the temperature 30℃. Through the domestication, sulfate reducting bacteria could grow under the conditions of the 90 mg/L of Cu2+ concentration and pH=4.5. Our results may provide the strains for the treatment of acidic mine drainage containing Cu2+
出处 《江西理工大学学报》 CAS 2013年第3期6-10,共5页 Journal of Jiangxi University of Science and Technology
基金 江西省科技支撑计划项目资助(20121BBG70004) 江西理工大学校级自选课题项目资助(jxxj11007)
关键词 硫酸盐还原菌 生长条件 酸性矿山废水 驯化 sulfate reducting bacteria growth conditions Acidic Mine Drainage domestication
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