摘要
目的探讨黄芩苷及联合左氧氟沙星(LFX)对金黄色葡萄球菌(S.a)生物被膜(BF)的影响。方法以临床分离S.a菌株17546为实验菌株,构建生物膜体外模型;分为空白组、黄芩苷组、LFX组、黄芩苷+LFX组;二倍稀释法测定药物的最低抑菌及杀菌浓度;连续稀释法行活菌计数;激光扫描共聚焦显微镜(CLSM)及扫描电镜(SEM)观察BF形态。结果 S.a17546培养3 d后可形成早期的BF。药物作用12 h后BF内活菌计数示:LFX、黄芩苷单独作用与空白组相比,差异无显著性(P>0.05);但与空白组、LFX单独作用组相比,黄芩苷+LFX组活菌计数均明显减少(P<0.05)。CLSM示黄芩苷+LFX组与空白组、LFX单独作用组相比,死菌明显增多。SEM示黄芩苷+LFX组与空白组、LFX单独作用组相比菌数及胞外基质均显著减少。结论黄芩苷在体外对S.a BF具有破坏作用,与LFX联合应用,可显著增强LFX对BF的杀菌作用。
【Objective】 To observe the effect of Baicalin in combination with Levofloxacin（LFX） on the Staphylococcus aureus（S.a） biofilm.【Methods】 The early model（3d） of S.a biofilm was established in vitro,which then were treated by different drugs： the control,baicalin,LFX and Baicalin＋LFX groups were observed under scanning electronic microscope（SEM） and confocal laser scanning microscope（CLSM）;the double dilution method was used to measure the minimum inhibitory concentration（MIC） and the minimum bactericidal concentration（MBC） of test drugs;the numbers of S.a in BF were counted by serial dilution method after 12h in test drugs.【Result】 Early（3d） S.a BF models were successfully established.Bacterial counts of S.a in BF,which were reacted in drugs 12 h,showed that： there were no significant discrepancy between the control group,baicalin and LFX groups（P ＆gt;0.05）;the bacterial number of Baicalin＋LFX group were significantly less than the control and LFX groups（P ＆lt;0.05）.【Conclusion】 Baicalin can destruct the biofilm of S.a and significantly enhance the bactericidal activities of LFX to S.a BF.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2013年第13期42-45,共4页
China Journal of Modern Medicine
基金
国家自然科学基金(No:81060002)
广西自然科学基金(No:2012GXNSFAA053095)
