摘要
目的了解天津市蓟县主要鼠类和媒介蚤的分布、种类、密度及其自然感染鼠疫菌和汉坦病毒(HV)情况,为有效预防控制鼠疫和肾综合征出血热(HFRS)提供依据。方法采用现场调查,以笼日法捕鼠,对捕获鼠类和检获蚤分类鉴定,计算鼠密度和带蚤率;采用间接血凝试验检测鼠疫菌F1抗体,用PCR方法检测鼠疫菌特异性基因fra和pla;采用直接免疫荧光法检测鼠肺HV抗原(HFRSAg)。结果2011年4-9月在蓟县共捕鼠269只,鼠密度为1.15%,隶属3科7属8种;检获不等单蚤9匹,染蚤率为3.68%,总蚤指数为0.055;采集鼠血清188份,鼠疫血清抗体检测,结果均为阴性;采集鼠肝脏247份,鼠疫核酸检测,结果均为阴性;采集鼠肺脏89份,汉坦病毒阳性1份,阳性率为1.12%,经鉴定为汉城型病毒。结论褐家鼠是蓟县的优势鼠种,鼠密度和蚤指数均低于鼠疫控制标准警戒线,未发现鼠间鼠疫流行迹象;HFRS的主要宿主为褐家鼠,检出汉城型病毒。
Objective To investigate the species, distribution, and density of dominant species of rodents and parasitical fleas and the infections of rodents with Yersinia pestis and hantavirus （HV） in Jixian of Tianjin, China, and to provide a basis for effective prevention and control of plague and hemorrhagic fever with renal syndrome （HFRS）. Methods Cages were used to capture rodents. The captured rodents and the fleas collected from the rodents were classified and identified, and the density of rodents and flea carrying rate were calculated. Indirect hemagglutination assay was used to detect the Y. pestis F1 antibody. Polymerase chain reaction was used to identify the specific genes of Y. pestis （fra and pla）. Fluorescent antibody technique was used to detect HV antigen in rodent lungs （HFRS Ag）. Results From April to September in 2011, 269 rodents （8 species, 7 genera, and 3 families） were captured in Jixian of Tianjin, and the density of rodents was 1.15%; 9 fleas （Monopsyllus anisus） were collected from the rodents; the flea carrying rate was 3.68% , and the total flea index was 0.055. A total of 188 serum samples of rodents were collected, and no Y. pestis F1 antibody was detected. A total of 247 liver samples of rodents were collected, and the specific genes of Y. pestis were not detected. A total of 89 lung samples of rodents were collected, and HV antigen was detected in one sample, with a positive rate of 1.12%; the HV was confirmed as Seoul virus （SEOV）. Conclusion R. norvegicus is the dominant species of rodents in Jixian of Tianjin. Both the rodent density and flea index are lower than the standard precaution level for plague control, and no rodent plague epidemic is found. R. norvegicus is the major host animal of HFRS, from which SEOV is detected.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2013年第3期257-259,共3页
Chinese Journal of Vector Biology and Control
关键词
鼠疫
肾综合征出血热
宿主动物
Plague
Hemorrhagic fever with renal syndrome
Host animal
