摘要
采用梯度浓度盐(NaCl)胁迫15个不同遗传背景的甘蓝型油菜自交系,于发芽期考查发芽率、发芽势、根长、胚轴长、单株鲜重和地上鲜重等6个性状。结果表明,随着盐浓度的增加,甘蓝型油菜的相对发芽率呈Boltz-mann曲线变化,214mmol/L NaCl为鉴定油菜品系耐盐性的最适浓度。在该浓度下,6个性状中根长和茎长受影响程度最大,发芽率、发芽势和总鲜重次之,地上鲜重影响最小。性状相对值之间存在显著正相关。根长和茎长可以作为评价油菜耐盐性的早期评价指标。聚类分析表明,15个品系可分为3类,其中WH126和WH129为最耐盐品系,WH137为盐敏感品系。
Fifteen Brassica napus genotypes were stressed by gradient NaCl concentrations.Six physiological characteristics including germination rate,germination potential,root length,hypocotyl length,plantlet weight and above ground weight were tested on germination stage.Results showed that relative germination rate changed in Boltzmann curve with various NaCl concentrations.214mmol/L was defined as the optimum salt concentration for evaluating the salt tolerance of rapeseed.Under this salt stress,root and hypocotyl lengths decreased remarkably,germination rate,germination potential and plantlet weight changed less,and above ground weight changed the least.Results showed significant correlations among the six characteristics.Root length and hypocotyl length could be used to forecast its salt-tolerance ability of a given line preliminarily.Cluster analysis indicated that the 15 lines could be divided into 3 groups.WH126 and WH129 had higher potential of high salt-tolerance.WH137 was the most salt-sensitive line.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2013年第3期271-275,共5页
Chinese Journal of Oil Crop Sciences
基金
农业部油料作物生物学重点实验室开放课题(2012-1)
江苏省农业科技自主创新资金[CX(12)2028]
关键词
发芽期
盐胁迫
甘蓝型油菜
耐盐性鉴定
Germinating stage
Stressed by sodium chloride
Brassica napus
Salt tolerance identification