摘要
以"丰香"草莓丛生芽为外植体,采用正交实验设计,研究了6-BA、琼脂、铵离子不同浓度组合对草莓试管苗玻璃化的影响,同时测定了玻璃化试管苗与正常苗的生理指标差异。结果表明:3种因素对草莓试管苗玻璃化均有显著影响,影响大小依次为6-BA>铵离子>琼脂;能有效降低试管苗玻璃化的组合为1.0mg/L 6-BA+6.0g/L琼脂+1 650mg/L铵离子,增殖倍数也较高;玻璃化苗的组织含水量和膜透性较正常苗高,可溶性糖含量则相反;玻璃化苗的叶绿素含量较正常苗显著降低,叶绿素a/叶绿素b在正常苗与玻璃苗间无显著差异,玻璃化苗较正常苗超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性降低,过氧化物酶(POD)活性升高。
Taking ＇Toyonoka＇ strawberry bud as explants,by method of orthogonal design,different combinations of 6-BA,agar,ammonium ion（ammonium chloride） were added to subculture medium of strawberry,the effects on vitrification of strawberry shoots were studied.Meanwhile,physiological and biochemical indexes between vitrification shoots and normal plantlets were determined and compared.The results showed that there were significant differences in the vitrification rate of shoots among 9 treatments.Analysis of variance showed that 6-BA was the most significant impactor on strawberry vitrification,the second was ammonium ion,and agar affected least.The optimum one was 1.0 g/L 6-BA＋6.0 g/L agar＋1 650 mg/L ammonium ion and the vitrification rate only was 26.22%,with higher proliferation ratio.Organization moisture content and membrane permeability of vitrification shoots were higher than normal plantlets,soluble sugar on the contrary.Chlorophyll content of vitrification shoots was significantly lower than normal plantlets,but there had no significant differences of Chla/Chlb between normal plantlets and vitrification shoots.Oxide dismutase（SOD） and catalase（CAT） activity of vitrification shoots were reduced compared with normal plantlets,while peroxidase（POD） activity increased.
出处
《北方园艺》
CAS
北大核心
2013年第13期131-134,共4页
Northern Horticulture
关键词
草莓试管苗
正交设计
玻璃化
生理生化
strawberry shoot
orthogonal design
vitrification
physiology and biochemistry
